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Literature summary extracted from

  • Watabe, S.; Makino, Y.; Ogawa, K.; Hiroi, T.; Yamamoto, Y.; Takahashi, S.Y.
    Mitochondrial thioredoxin reductase in bovine adrenal cortex its purification, properties, nucleotide/amino acid sequences, and identification of selenocysteine (1999), Eur. J. Biochem., 264, 74-84.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.8.1.9 cysteine C-terminal redox active selenocysteine motif Bos taurus
1.8.1.9 EDTA 4% activity remaining without Bos taurus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.8.1.9 amino acid and DNA sequence analysis Bos taurus

General Stability

EC Number General Stability Organism
1.8.1.9 4% activity remaining without EDTA Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.8.1.9 1-chloro-2,4-dinitrobenzene i.e. DNCB; mitochondrial isoform Bos taurus
1.8.1.9 Ca2+
-
Bos taurus
1.8.1.9 Ca2+
-
Escherichia coli
1.8.1.9 Cu2+ strong inhibition Bos taurus
1.8.1.9 Fe2+ strong inhibition Bos taurus
1.8.1.9 Mg2+ slightly Bos taurus
1.8.1.9 Mn2+ strong inhibition Bos taurus
1.8.1.9 Zn2+ strong inhibition Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.1.9 0.0028
-
thioredoxin
-
Escherichia coli
1.8.1.9 0.0028
-
thioredoxin
-
Bos taurus
1.8.1.9 0.006
-
thioredoxin human thioredoxin Bos taurus
1.8.1.9 0.07
-
thioredoxin
-
Escherichia coli
1.8.1.9 0.07
-
thioredoxin E. coli thioredoxin Bos taurus
1.8.1.9 0.62
-
selenocysteine
-
Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.8.1.9 cytosol
-
Bos taurus 5829
-
1.8.1.9 mitochondrion
-
Bos taurus 5739
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.8.1.9 selenocysteine contains selenocysteine as part of the redox active selenosulfide in the active center Bos taurus
1.8.1.9 selenocysteine encoded by TGA stop codon Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.8.1.9 56000
-
2 * 56000, SDS-PAGE Bos taurus
1.8.1.9 100000
-
gel filtration Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
1.8.1.9 Bos taurus
-
cytosolic and mitochondrial isoforms
-
1.8.1.9 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.1.9 mitochondrial isoform Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.8.1.9 adrenal gland cortex Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.8.1.9 13.9
-
mitochondrial isoform Bos taurus
1.8.1.9 22
-
purified enzyme, coupled assay with DTNB Escherichia coli
1.8.1.9 22
-
purified enzyme, coupled assay with DTNB Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.1.9 5,5'-dithiobis(2-nitrobenzoic acid) + NADPH i.e. DTNB Escherichia coli 2-nitro-5-thiobenzoate + NADP+
-
?
1.8.1.9 5,5'-dithiobis(2-nitrobenzoic acid) + NADPH i.e. DTNB Bos taurus 2-nitro-5-thiobenzoate + NADP+
-
?
1.8.1.9 5,5'-dithiobis(2-nitrobenzoic acid) + NADPH coupled assay Escherichia coli 2-nitro-5-thiobenzoate + NADP+
-
?
1.8.1.9 5,5'-dithiobis(2-nitrobenzoic acid) + NADPH coupled assay Bos taurus 2-nitro-5-thiobenzoate + NADP+
-
?
1.8.1.9 dithiothreitol + NADPH no activity Bos taurus ?
-
?
1.8.1.9 GSSG + NADPH no activity Bos taurus GSH + NADP+
-
?
1.8.1.9 oxidized lipoate + NADPH no activity with DL-alpha-lipoate Bos taurus ?
-
?
1.8.1.9 selenocysteine + NADPH
-
Bos taurus selenocystine + NADP+
-
?
1.8.1.9 thioredoxin + NADP+ coupled assay with DTNB Escherichia coli thioredoxin disulfide + NADPH
-
?
1.8.1.9 thioredoxin + NADP+ coupled assay with DTNB Bos taurus thioredoxin disulfide + NADPH
-
?
1.8.1.9 thioredoxin + NADP+
-
Escherichia coli thioredoxin disulfide + NADPH + H+
-
?
1.8.1.9 thioredoxin + NADP+ no activity Bos taurus thioredoxin disulfide + NADPH + H+
-
?

Subunits

EC Number Subunits Comment Organism
1.8.1.9 dimer
-
Escherichia coli
1.8.1.9 dimer 2 * 56000, SDS-PAGE Bos taurus

Synonyms

EC Number Synonyms Comment Organism
1.8.1.9 More 2 classes of thioredoxin reductases: high molecular weight or large proteins e.g. in mammalia, Plasmodium falsiparum and Caenorhabditis elegans, and low molecular weight proteins e.g. in bacteria Bos taurus
1.8.1.9 TR
-
Escherichia coli
1.8.1.9 TR
-
Bos taurus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.8.1.9 37
-
assay at Bos taurus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.8.1.9 1030
-
thioredoxin human thioredoxin Bos taurus
1.8.1.9 1200
-
thioredoxin
-
Bos taurus
1.8.1.9 1300
-
thioredoxin thioredoxin from E. coli Bos taurus
1.8.1.9 1600
-
selenocysteine
-
Bos taurus

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.1.9 NADPH
-
Escherichia coli
1.8.1.9 NADPH
-
Bos taurus