EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.8.1.9 | cysteine | C-terminal redox active selenocysteine motif | Bos taurus | |
1.8.1.9 | EDTA | 4% activity remaining without | Bos taurus |
EC Number | Cloned (Comment) | Organism |
---|---|---|
1.8.1.9 | amino acid and DNA sequence analysis | Bos taurus |
EC Number | General Stability | Organism |
---|---|---|
1.8.1.9 | 4% activity remaining without EDTA | Bos taurus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.8.1.9 | 1-chloro-2,4-dinitrobenzene | i.e. DNCB; mitochondrial isoform | Bos taurus | |
1.8.1.9 | Ca2+ | - |
Bos taurus | |
1.8.1.9 | Ca2+ | - |
Escherichia coli | |
1.8.1.9 | Cu2+ | strong inhibition | Bos taurus | |
1.8.1.9 | Fe2+ | strong inhibition | Bos taurus | |
1.8.1.9 | Mg2+ | slightly | Bos taurus | |
1.8.1.9 | Mn2+ | strong inhibition | Bos taurus | |
1.8.1.9 | Zn2+ | strong inhibition | Bos taurus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.8.1.9 | 0.0028 | - |
thioredoxin | - |
Escherichia coli | |
1.8.1.9 | 0.0028 | - |
thioredoxin | - |
Bos taurus | |
1.8.1.9 | 0.006 | - |
thioredoxin | human thioredoxin | Bos taurus | |
1.8.1.9 | 0.07 | - |
thioredoxin | - |
Escherichia coli | |
1.8.1.9 | 0.07 | - |
thioredoxin | E. coli thioredoxin | Bos taurus | |
1.8.1.9 | 0.62 | - |
selenocysteine | - |
Bos taurus |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.8.1.9 | cytosol | - |
Bos taurus | 5829 | - |
1.8.1.9 | mitochondrion | - |
Bos taurus | 5739 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.8.1.9 | selenocysteine | contains selenocysteine as part of the redox active selenosulfide in the active center | Bos taurus | |
1.8.1.9 | selenocysteine | encoded by TGA stop codon | Bos taurus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.8.1.9 | 56000 | - |
2 * 56000, SDS-PAGE | Bos taurus |
1.8.1.9 | 100000 | - |
gel filtration | Bos taurus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.8.1.9 | Bos taurus | - |
cytosolic and mitochondrial isoforms | - |
1.8.1.9 | Escherichia coli | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.8.1.9 | mitochondrial isoform | Bos taurus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.8.1.9 | adrenal gland | cortex | Bos taurus | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.8.1.9 | 13.9 | - |
mitochondrial isoform | Bos taurus |
1.8.1.9 | 22 | - |
purified enzyme, coupled assay with DTNB | Escherichia coli |
1.8.1.9 | 22 | - |
purified enzyme, coupled assay with DTNB | Bos taurus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.8.1.9 | 5,5'-dithiobis(2-nitrobenzoic acid) + NADPH | i.e. DTNB | Escherichia coli | 2-nitro-5-thiobenzoate + NADP+ | - |
? | |
1.8.1.9 | 5,5'-dithiobis(2-nitrobenzoic acid) + NADPH | i.e. DTNB | Bos taurus | 2-nitro-5-thiobenzoate + NADP+ | - |
? | |
1.8.1.9 | 5,5'-dithiobis(2-nitrobenzoic acid) + NADPH | coupled assay | Escherichia coli | 2-nitro-5-thiobenzoate + NADP+ | - |
? | |
1.8.1.9 | 5,5'-dithiobis(2-nitrobenzoic acid) + NADPH | coupled assay | Bos taurus | 2-nitro-5-thiobenzoate + NADP+ | - |
? | |
1.8.1.9 | dithiothreitol + NADPH | no activity | Bos taurus | ? | - |
? | |
1.8.1.9 | GSSG + NADPH | no activity | Bos taurus | GSH + NADP+ | - |
? | |
1.8.1.9 | oxidized lipoate + NADPH | no activity with DL-alpha-lipoate | Bos taurus | ? | - |
? | |
1.8.1.9 | selenocysteine + NADPH | - |
Bos taurus | selenocystine + NADP+ | - |
? | |
1.8.1.9 | thioredoxin + NADP+ | coupled assay with DTNB | Escherichia coli | thioredoxin disulfide + NADPH | - |
? | |
1.8.1.9 | thioredoxin + NADP+ | coupled assay with DTNB | Bos taurus | thioredoxin disulfide + NADPH | - |
? | |
1.8.1.9 | thioredoxin + NADP+ | - |
Escherichia coli | thioredoxin disulfide + NADPH + H+ | - |
? | |
1.8.1.9 | thioredoxin + NADP+ | no activity | Bos taurus | thioredoxin disulfide + NADPH + H+ | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.8.1.9 | dimer | - |
Escherichia coli |
1.8.1.9 | dimer | 2 * 56000, SDS-PAGE | Bos taurus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.8.1.9 | More | 2 classes of thioredoxin reductases: high molecular weight or large proteins e.g. in mammalia, Plasmodium falsiparum and Caenorhabditis elegans, and low molecular weight proteins e.g. in bacteria | Bos taurus |
1.8.1.9 | TR | - |
Escherichia coli |
1.8.1.9 | TR | - |
Bos taurus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.8.1.9 | 37 | - |
assay at | Bos taurus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.8.1.9 | 1030 | - |
thioredoxin | human thioredoxin | Bos taurus | |
1.8.1.9 | 1200 | - |
thioredoxin | - |
Bos taurus | |
1.8.1.9 | 1300 | - |
thioredoxin | thioredoxin from E. coli | Bos taurus | |
1.8.1.9 | 1600 | - |
selenocysteine | - |
Bos taurus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.8.1.9 | NADPH | - |
Escherichia coli | |
1.8.1.9 | NADPH | - |
Bos taurus |