BRENDA - Enzyme Database

Mammalian thioredoxin reductase is irreversibly inhibited by dinitrohalobenzenes by alkylation of both the redox active selenocysteine and its neighboring cysteine residue

Nordberg, J.; Zhong, L.; Holmgren, A.; Arner, E.S.J.; J. Biol. Chem. 273, 10835-10842 (1998)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.8.1.9
1-chloro-2,4-dinitrobenzene
i.e. DNCB; irreversible, with NADPH, alkylation of the active site cysteine disulfide, strong increase in oxidation activity of the enzyme against NADPH
Bos taurus
1.8.1.9
1-chloro-2,4-dinitrobenzene
i.e. DNCB; irreversible, with NADPH, alkylation of the active site cysteine disulfide, strong increase in oxidation activity of the enzyme against NADPH
Homo sapiens
1.8.1.9
1-Fluoro-2,4-dinitrobenzene
irreversible, with NADPH, alkylation of the active site cysteine disulfide, strong increase in oxidation activity of the enzyme against NADPH
Bos taurus
1.8.1.9
1-Fluoro-2,4-dinitrobenzene
irreversible, with NADPH, alkylation of the active site cysteine disulfide, strong increase in oxidation activity of the enzyme against NADPH
Homo sapiens
1.8.1.9
4-Vinylpyridine
irreversible
Bos taurus
1.8.1.9
4-Vinylpyridine
irreversible
Homo sapiens
1.8.1.9
iodoacetate
irreversible
Bos taurus
1.8.1.9
iodoacetate
irreversible
Homo sapiens
1.8.1.9
additional information
no inhibition with dinitrohalobenzene analogues: 1,4-dichlorobenzene, 1-chloro-4-nitrobenzene, 1-chloro-3,4-dinitrobenzene, 1-chloro-2,5-dinitrobenzene
Bos taurus
1.8.1.9
additional information
no inhibition with dinitrohalobenzene analogues: 1,4-dichlorobenzene, 1-chloro-4-nitrobenzene, 1-chloro-3,4-dinitrobenzene, 1-chloro-2,5-dinitrobenzene
Homo sapiens
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.8.1.9
120000
-
-
Bos taurus
1.8.1.9
120000
-
-
Homo sapiens
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.8.1.9
Bos taurus
-
-
-
1.8.1.9
Homo sapiens
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.8.1.9
-
Bos taurus
1.8.1.9
-
Homo sapiens
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.8.1.9
placenta
-
Homo sapiens
-
1.8.1.9
thymus
-
Bos taurus
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.8.1.9
5,5'-dithiobis(2-nitrobenzoic acid) + NADPH
i.e. DTNB
394944
Homo sapiens
2-nitro-5-thiobenzoate + NADP+
-
-
-
?
1.8.1.9
5,5'-dithiobis(2-nitrobenzoic acid) + NADPH
i.e. DTNB
394944
Bos taurus
2-nitro-5-thiobenzoate + NADP+
-
-
-
?
1.8.1.9
5,5'-dithiobis(2-nitrobenzoic acid) + NADPH
coupled assay
394944
Homo sapiens
2-nitro-5-thiobenzoate + NADP+
-
-
-
?
1.8.1.9
5,5'-dithiobis(2-nitrobenzoic acid) + NADPH
coupled assay
394944
Bos taurus
2-nitro-5-thiobenzoate + NADP+
-
-
-
?
1.8.1.9
thioredoxin + NADP+
coupled assay with DTNB
394944
Homo sapiens
thioredoxin disulfide + NADPH
-
-
-
?
1.8.1.9
thioredoxin + NADP+
coupled assay with DTNB
394944
Bos taurus
thioredoxin disulfide + NADPH
-
-
-
?
1.8.1.9
thioredoxin + NADP+
coupled assay, measurement of NADPH oxidation in presence of insulin and thioredoxin
394944
Homo sapiens
thioredoxin disulfide + NADPH
-
-
-
?
1.8.1.9
thioredoxin + NADP+
coupled assay, measurement of NADPH oxidation in presence of insulin and thioredoxin
394944
Bos taurus
thioredoxin disulfide + NADPH
-
-
-
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.8.1.9
20
-
assay at
Bos taurus
1.8.1.9
20
-
assay at
Homo sapiens
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.8.1.9
8
-
assay at
Bos taurus
1.8.1.9
8
-
assay at
Homo sapiens
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.8.1.9
FAD
-
Homo sapiens
1.8.1.9
NADPH
-
Bos taurus
1.8.1.9
NADPH
-
Homo sapiens
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.8.1.9
FAD
-
Homo sapiens
1.8.1.9
NADPH
-
Bos taurus
1.8.1.9
NADPH
-
Homo sapiens
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.8.1.9
1-chloro-2,4-dinitrobenzene
i.e. DNCB; irreversible, with NADPH, alkylation of the active site cysteine disulfide, strong increase in oxidation activity of the enzyme against NADPH
Bos taurus
1.8.1.9
1-chloro-2,4-dinitrobenzene
i.e. DNCB; irreversible, with NADPH, alkylation of the active site cysteine disulfide, strong increase in oxidation activity of the enzyme against NADPH
Homo sapiens
1.8.1.9
1-Fluoro-2,4-dinitrobenzene
irreversible, with NADPH, alkylation of the active site cysteine disulfide, strong increase in oxidation activity of the enzyme against NADPH
Bos taurus
1.8.1.9
1-Fluoro-2,4-dinitrobenzene
irreversible, with NADPH, alkylation of the active site cysteine disulfide, strong increase in oxidation activity of the enzyme against NADPH
Homo sapiens
1.8.1.9
4-Vinylpyridine
irreversible
Bos taurus
1.8.1.9
4-Vinylpyridine
irreversible
Homo sapiens
1.8.1.9
iodoacetate
irreversible
Bos taurus
1.8.1.9
iodoacetate
irreversible
Homo sapiens
1.8.1.9
additional information
no inhibition with dinitrohalobenzene analogues: 1,4-dichlorobenzene, 1-chloro-4-nitrobenzene, 1-chloro-3,4-dinitrobenzene, 1-chloro-2,5-dinitrobenzene
Bos taurus
1.8.1.9
additional information
no inhibition with dinitrohalobenzene analogues: 1,4-dichlorobenzene, 1-chloro-4-nitrobenzene, 1-chloro-3,4-dinitrobenzene, 1-chloro-2,5-dinitrobenzene
Homo sapiens
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.8.1.9
120000
-
-
Bos taurus
1.8.1.9
120000
-
-
Homo sapiens
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.8.1.9
-
Bos taurus
1.8.1.9
-
Homo sapiens
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.8.1.9
placenta
-
Homo sapiens
-
1.8.1.9
thymus
-
Bos taurus
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.8.1.9
5,5'-dithiobis(2-nitrobenzoic acid) + NADPH
i.e. DTNB
394944
Homo sapiens
2-nitro-5-thiobenzoate + NADP+
-
-
-
?
1.8.1.9
5,5'-dithiobis(2-nitrobenzoic acid) + NADPH
i.e. DTNB
394944
Bos taurus
2-nitro-5-thiobenzoate + NADP+
-
-
-
?
1.8.1.9
5,5'-dithiobis(2-nitrobenzoic acid) + NADPH
coupled assay
394944
Homo sapiens
2-nitro-5-thiobenzoate + NADP+
-
-
-
?
1.8.1.9
5,5'-dithiobis(2-nitrobenzoic acid) + NADPH
coupled assay
394944
Bos taurus
2-nitro-5-thiobenzoate + NADP+
-
-
-
?
1.8.1.9
thioredoxin + NADP+
coupled assay with DTNB
394944
Homo sapiens
thioredoxin disulfide + NADPH
-
-
-
?
1.8.1.9
thioredoxin + NADP+
coupled assay with DTNB
394944
Bos taurus
thioredoxin disulfide + NADPH
-
-
-
?
1.8.1.9
thioredoxin + NADP+
coupled assay, measurement of NADPH oxidation in presence of insulin and thioredoxin
394944
Homo sapiens
thioredoxin disulfide + NADPH
-
-
-
?
1.8.1.9
thioredoxin + NADP+
coupled assay, measurement of NADPH oxidation in presence of insulin and thioredoxin
394944
Bos taurus
thioredoxin disulfide + NADPH
-
-
-
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.8.1.9
20
-
assay at
Bos taurus
1.8.1.9
20
-
assay at
Homo sapiens
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.8.1.9
8
-
assay at
Bos taurus
1.8.1.9
8
-
assay at
Homo sapiens