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Literature summary extracted from

  • Oblong, J.E.; Gasdaska, P.Y.; Sherrill, K.; Powis, G.
    Purification of human thioredoxin reductase: properties and characterization by absorption and circular dichroism spectroscopy (1993), Biochemistry, 32, 7271-7277.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.8.1.9 tightly bound FAD is not replaced during heat treatment or guanidinium hydrochloride Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.1.9 additional information
-
additional information
-
Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.8.1.9 60000
-
2 * 60000, SDS-PAGE Homo sapiens
1.8.1.9 130000 160000 sucrose density gradient centrifugation and gel filtration Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.8.1.9 thioredoxin + NADP+ Homo sapiens
-
thioredoxin disulfide + NADPH
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.8.1.9 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.1.9
-
Homo sapiens

Reaction

EC Number Reaction Comment Organism Reaction ID
1.8.1.9 thioredoxin + NADP+ = thioredoxin disulfide + NADPH + H+ three-dimensional structure Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.8.1.9 placenta
-
Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.8.1.9 33.8
-
purified enzyme Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.1.9 additional information redox system for electron transfer in the complex of apoenzyme, FAD and thioredoxin with NADPH or other electron acceptors Homo sapiens ?
-
?
1.8.1.9 thioredoxin + NADP+
-
Homo sapiens thioredoxin disulfide + NADPH
-
?
1.8.1.9 thioredoxin + NADP+ coupled assay, measurement of NADPH oxidation in presence of insulin and thioredoxin Homo sapiens thioredoxin disulfide + NADPH
-
?
1.8.1.9 thioredoxin + NADP+ in presence of NADPH, coupled assay Homo sapiens thioredoxin disulfide + NADPH + H+
-
?
1.8.1.9 thioredoxin + NADP+ i.e. DTNB Homo sapiens thioredoxin disulfide + NADPH + H+
-
?

Subunits

EC Number Subunits Comment Organism
1.8.1.9 dimer 2 * 60000, SDS-PAGE Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
1.8.1.9 TR
-
Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.8.1.9 40
-
reduction of insulin Homo sapiens
1.8.1.9 52
-
reduction of DTNB Homo sapiens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.8.1.9 60
-
1 h, no loss of activity Homo sapiens
1.8.1.9 65
-
above, irreversible loss of activity, no displacement of tightly bound FAD Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.1.9 FAD
-
Homo sapiens
1.8.1.9 NADPH
-
Homo sapiens