EC Number | General Stability | Organism |
---|---|---|
1.8.1.9 | tightly bound FAD is not replaced during heat treatment or guanidinium hydrochloride | Homo sapiens |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.8.1.9 | additional information | - |
additional information | - |
Homo sapiens |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.8.1.9 | 60000 | - |
2 * 60000, SDS-PAGE | Homo sapiens |
1.8.1.9 | 130000 | 160000 | sucrose density gradient centrifugation and gel filtration | Homo sapiens |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.8.1.9 | thioredoxin + NADP+ | Homo sapiens | - |
thioredoxin disulfide + NADPH | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.8.1.9 | Homo sapiens | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.8.1.9 | - |
Homo sapiens |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
1.8.1.9 | thioredoxin + NADP+ = thioredoxin disulfide + NADPH + H+ | three-dimensional structure | Homo sapiens |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.8.1.9 | placenta | - |
Homo sapiens | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.8.1.9 | 33.8 | - |
purified enzyme | Homo sapiens |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.8.1.9 | additional information | redox system for electron transfer in the complex of apoenzyme, FAD and thioredoxin with NADPH or other electron acceptors | Homo sapiens | ? | - |
? | |
1.8.1.9 | thioredoxin + NADP+ | - |
Homo sapiens | thioredoxin disulfide + NADPH | - |
? | |
1.8.1.9 | thioredoxin + NADP+ | coupled assay, measurement of NADPH oxidation in presence of insulin and thioredoxin | Homo sapiens | thioredoxin disulfide + NADPH | - |
? | |
1.8.1.9 | thioredoxin + NADP+ | in presence of NADPH, coupled assay | Homo sapiens | thioredoxin disulfide + NADPH + H+ | - |
? | |
1.8.1.9 | thioredoxin + NADP+ | i.e. DTNB | Homo sapiens | thioredoxin disulfide + NADPH + H+ | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.8.1.9 | dimer | 2 * 60000, SDS-PAGE | Homo sapiens |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.8.1.9 | TR | - |
Homo sapiens |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.8.1.9 | 40 | - |
reduction of insulin | Homo sapiens |
1.8.1.9 | 52 | - |
reduction of DTNB | Homo sapiens |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.8.1.9 | 60 | - |
1 h, no loss of activity | Homo sapiens |
1.8.1.9 | 65 | - |
above, irreversible loss of activity, no displacement of tightly bound FAD | Homo sapiens |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.8.1.9 | FAD | - |
Homo sapiens | |
1.8.1.9 | NADPH | - |
Homo sapiens |