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Literature summary extracted from
- Arabidopsis thaliana NADPH thioredoxin reductase. cDNA characterization and expression of the recombinant protein in Escherichia coli (1994), J. Mol. Biol., 235, 1357-1363.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.8.1.9 | cysteine | part of the active site | Arabidopsis thaliana |  |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.8.1.9 | strong overexpression in Escherichia coli, DNA sequence analysis | Arabidopsis thaliana |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.8.1.9 | additional information | - |
additional information | additionally to the Arabidopsis thaliana thioredoxin, several other thioredoxins have been tested, Km-values | Arabidopsis thaliana | |
| 1.8.1.9 | 0.0011 | - |
thioredoxin | recombinant enzyme | Arabidopsis thaliana | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.8.1.9 | cytosol | - |
Arabidopsis thaliana | 5829 | - |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.8.1.9 | Arabidopsis thaliana | - |
EMBL accession numbers Z23109, Z23108 | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.8.1.9 | recombinant from Escherichia coli | Arabidopsis thaliana |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.8.1.9 | thioredoxin + NADP+ | - |
Arabidopsis thaliana | thioredoxin disulfide + NADPH | - |
? | |
| 1.8.1.9 | thioredoxin + NADP+ | i.e. DTNB | Arabidopsis thaliana | thioredoxin disulfide + NADPH + H+ | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.8.1.9 | dimer | 2 * 35000-35328, SDS-PAGE and amino acid analysis | Arabidopsis thaliana |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.8.1.9 | 60 | - |
5 min, active | Arabidopsis thaliana |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.8.1.9 | FAD | - |
Arabidopsis thaliana | |
| 1.8.1.9 | NADPH | specific for | Arabidopsis thaliana | |
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