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Enzymatic synthesis of desoxyribonucleotides. V. Purification and properties of thioredoxin reductase from Escherichia coli B

Moore, E.C.; Reichard, P.; Thelander, L.; J. Biol. Chem. 239, 3445-3452 (1964)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
1.8.1.9
KCN
-
Escherichia coli
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.8.1.9
arsenite
-
Escherichia coli
1.8.1.9
CdCl2
-
Escherichia coli
1.8.1.9
p-mercuribenzoate
-
Escherichia coli
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.8.1.9
68000
-
sedimentation equilibrium, ultracentrifugation
Escherichia coli
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.8.1.9
Escherichia coli
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.8.1.9
-
Escherichia coli
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.8.1.9
additional information
-
-
Escherichia coli
1.8.1.9
58
-
purified enzyme
Escherichia coli
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.8.1.9
additional information
slowly reduces other proteins, e.g. insulin, lipoate and ribonuclease
394931
Escherichia coli
?
-
-
-
-
1.8.1.9
thioredoxin + NADP+
-
394931
Escherichia coli
thioredoxin disulfide + NADPH
-
394931
Escherichia coli
ir
1.8.1.9
thioredoxin + NADP+
-
394931
Escherichia coli
thioredoxin disulfide + NADPH + H+
-
394931
Escherichia coli
ir
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.8.1.9
65
-
7 min, active
Escherichia coli
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.8.1.9
7
-
assay at
Escherichia coli
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.8.1.9
FAD
-
Escherichia coli
1.8.1.9
NADPH
-
Escherichia coli
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
1.8.1.9
KCN
-
Escherichia coli
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.8.1.9
FAD
-
Escherichia coli
1.8.1.9
NADPH
-
Escherichia coli
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.8.1.9
arsenite
-
Escherichia coli
1.8.1.9
CdCl2
-
Escherichia coli
1.8.1.9
p-mercuribenzoate
-
Escherichia coli
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.8.1.9
68000
-
sedimentation equilibrium, ultracentrifugation
Escherichia coli
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.8.1.9
-
Escherichia coli
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.8.1.9
additional information
-
-
Escherichia coli
1.8.1.9
58
-
purified enzyme
Escherichia coli
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.8.1.9
additional information
slowly reduces other proteins, e.g. insulin, lipoate and ribonuclease
394931
Escherichia coli
?
-
-
-
-
1.8.1.9
thioredoxin + NADP+
-
394931
Escherichia coli
thioredoxin disulfide + NADPH
-
394931
Escherichia coli
ir
1.8.1.9
thioredoxin + NADP+
-
394931
Escherichia coli
thioredoxin disulfide + NADPH + H+
-
394931
Escherichia coli
ir
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.8.1.9
65
-
7 min, active
Escherichia coli
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.8.1.9
7
-
assay at
Escherichia coli