BRENDA - Enzyme Database show

Rat liver thioredoxin and thioredoxin reductase: purification and characterization

Luthman, M.; Holmgren, A.; Biochemistry 21, 6628-6633 (1982)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
1.8.1.9
cysteine
part of the active site
Rattus norvegicus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.8.1.9
arsenite
-
Rattus norvegicus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.8.1.9
0.0025
-
reductase
thioredoxin from rat, bovine substrate
Rattus norvegicus
1.8.1.9
0.0025
-
thioredoxin
substrate: thioredoxin from calf and rat
Rattus norvegicus
1.8.1.9
0.006
-
NADPH
-
Rattus norvegicus
1.8.1.9
0.045
-
thioredoxin
substrate: thioredoxin from E. coli
Rattus norvegicus
1.8.1.9
0.66
-
DTNB
-
Rattus norvegicus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.8.1.9
58000
-
2 * 58000, SDS-PAGE
Rattus norvegicus
1.8.1.9
116000
-
gel filtration
Rattus norvegicus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.8.1.9
Rattus norvegicus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.8.1.9
-
Rattus norvegicus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.8.1.9
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.8.1.9
35
-
purified enzyme, coupled assay with DTNB
Rattus norvegicus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.8.1.9
5,5'-dithiobis(2-nitrobenzoic acid) + NADPH
i.e. DTNB
394921
Rattus norvegicus
2-nitro-5-thiobenzoate + NADP+
-
-
-
?
1.8.1.9
5,5'-dithiobis(2-nitrobenzoic acid) + NADPH
coupled assay
394921
Rattus norvegicus
2-nitro-5-thiobenzoate + NADP+
-
-
-
?
1.8.1.9
alloxan + NADPH
-
394921
Rattus norvegicus
?
-
-
-
?
1.8.1.9
L-cysteine + NADPH
-
394921
Rattus norvegicus
L-cystine + NADP+
-
-
-
?
1.8.1.9
menadione + NADPH
-
394921
Rattus norvegicus
?
-
-
-
?
1.8.1.9
oxidized lipoamide + NADPH
-
394921
Rattus norvegicus
lipoamide disulfide + NADP+
-
-
-
?
1.8.1.9
thioredoxin + NADP+
-
394921
Rattus norvegicus
thioredoxin disulfide + NADPH
-
394921
Rattus norvegicus
ir
1.8.1.9
thioredoxin + NADP+
-
394921
Rattus norvegicus
thioredoxin disulfide + NADPH + H+
-
394921
Rattus norvegicus
ir
Subunits
EC Number
Subunits
Commentary
Organism
1.8.1.9
dimer
2 * 58000, SDS-PAGE
Rattus norvegicus
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.8.1.9
50
-
thioredoxin
-
Rattus norvegicus
1.8.1.9
66.7
-
DTNB
-
Rattus norvegicus
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.8.1.9
FAD
-
Rattus norvegicus
1.8.1.9
NADPH
-
Rattus norvegicus
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
1.8.1.9
cysteine
part of the active site
Rattus norvegicus
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.8.1.9
FAD
-
Rattus norvegicus
1.8.1.9
NADPH
-
Rattus norvegicus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.8.1.9
arsenite
-
Rattus norvegicus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.8.1.9
0.0025
-
reductase
thioredoxin from rat, bovine substrate
Rattus norvegicus
1.8.1.9
0.0025
-
thioredoxin
substrate: thioredoxin from calf and rat
Rattus norvegicus
1.8.1.9
0.006
-
NADPH
-
Rattus norvegicus
1.8.1.9
0.045
-
thioredoxin
substrate: thioredoxin from E. coli
Rattus norvegicus
1.8.1.9
0.66
-
DTNB
-
Rattus norvegicus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.8.1.9
58000
-
2 * 58000, SDS-PAGE
Rattus norvegicus
1.8.1.9
116000
-
gel filtration
Rattus norvegicus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.8.1.9
-
Rattus norvegicus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.8.1.9
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.8.1.9
35
-
purified enzyme, coupled assay with DTNB
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.8.1.9
5,5'-dithiobis(2-nitrobenzoic acid) + NADPH
i.e. DTNB
394921
Rattus norvegicus
2-nitro-5-thiobenzoate + NADP+
-
-
-
?
1.8.1.9
5,5'-dithiobis(2-nitrobenzoic acid) + NADPH
coupled assay
394921
Rattus norvegicus
2-nitro-5-thiobenzoate + NADP+
-
-
-
?
1.8.1.9
alloxan + NADPH
-
394921
Rattus norvegicus
?
-
-
-
?
1.8.1.9
L-cysteine + NADPH
-
394921
Rattus norvegicus
L-cystine + NADP+
-
-
-
?
1.8.1.9
menadione + NADPH
-
394921
Rattus norvegicus
?
-
-
-
?
1.8.1.9
oxidized lipoamide + NADPH
-
394921
Rattus norvegicus
lipoamide disulfide + NADP+
-
-
-
?
1.8.1.9
thioredoxin + NADP+
-
394921
Rattus norvegicus
thioredoxin disulfide + NADPH
-
394921
Rattus norvegicus
ir
1.8.1.9
thioredoxin + NADP+
-
394921
Rattus norvegicus
thioredoxin disulfide + NADPH + H+
-
394921
Rattus norvegicus
ir
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.8.1.9
dimer
2 * 58000, SDS-PAGE
Rattus norvegicus
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.8.1.9
50
-
thioredoxin
-
Rattus norvegicus
1.8.1.9
66.7
-
DTNB
-
Rattus norvegicus