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Literature summary extracted from

  • Clement-Metral, J.D.; Höög, J.O.; Holmgren, A.
    Characterization of the thioredoxin system in the facultative phototroph Rhodobacter sphaeroides Y [published erratum appears in Eur J Biochem 1987 May 4;164(3):713] (1986), Eur. J. Biochem., 161, 119-126.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.8.1.9 cysteine part of the active site Cereibacter sphaeroides

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.1.9 0.003
-
 thioredoxin
-
 Cereibacter sphaeroides
1.8.1.9 0.0033
-
 reductase
-
 Cereibacter sphaeroides
1.8.1.9 0.0144
-
 thioredoxin substrate: thioredoxin from E. coli Cereibacter sphaeroides

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.8.1.9 34000
-
 2 * 34000, SDS-PAGE Cereibacter sphaeroides
1.8.1.9 68000
-
 native PAGE Cereibacter sphaeroides

Organism

EC Number Organism UniProt Comment Textmining
1.8.1.9 Cereibacter sphaeroides
-
 Y
-
1.8.1.9 Cereibacter sphaeroides Y
-
 Y
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.1.9
-
 Cereibacter sphaeroides

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.8.1.9 additional information
-
-
 Cereibacter sphaeroides

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.1.9 5,5'-dithiobis(2-nitrobenzoic acid) + NADPH i.e. DTNB Cereibacter sphaeroides 2-nitro-5-thiobenzoate + NADP+
-
 ?
1.8.1.9 5,5'-dithiobis(2-nitrobenzoic acid) + NADPH requires thioredoxin for reduction of DTNB Cereibacter sphaeroides 2-nitro-5-thiobenzoate + NADP+
-
 ?
1.8.1.9 5,5'-dithiobis(2-nitrobenzoic acid) + NADPH i.e. DTNB Cereibacter sphaeroides Y 2-nitro-5-thiobenzoate + NADP+
-
 ?
1.8.1.9 5,5'-dithiobis(2-nitrobenzoic acid) + NADPH requires thioredoxin for reduction of DTNB Cereibacter sphaeroides Y 2-nitro-5-thiobenzoate + NADP+
-
 ?
1.8.1.9 additional information
-
 Cereibacter sphaeroides ?
-
 ?
1.8.1.9 additional information
-
 Cereibacter sphaeroides Y ?
-
 ?
1.8.1.9 thioredoxin + NADP+
-
 Cereibacter sphaeroides thioredoxin disulfide + NADPH
-
 r
1.8.1.9 thioredoxin + NADP+
-
 Cereibacter sphaeroides Y thioredoxin disulfide + NADPH
-
 r
1.8.1.9 thioredoxin + NADP+ in presence of NADPH, coupled assay Cereibacter sphaeroides thioredoxin disulfide + NADPH + H+
-
 r
1.8.1.9 thioredoxin + NADP+ in presence of NADPH, coupled assay Cereibacter sphaeroides Y thioredoxin disulfide + NADPH + H+
-
 r

Subunits

EC Number Subunits Comment Organism
1.8.1.9 dimer 2 * 34000, SDS-PAGE Cereibacter sphaeroides

Synonyms

EC Number Synonyms Comment Organism
1.8.1.9 TRase
-
 Cereibacter sphaeroides

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.1.9 FAD 2 FAD per dimer Cereibacter sphaeroides
1.8.1.9 NADPH strictly dependent on NADPH, inactive with NADH Cereibacter sphaeroides