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Literature summary extracted from

  • Russel, M.; Model, P.
    Direct cloning of the trxB gene that encodes thioredoxin reductase (1985), J. Bacteriol., 163, 238-242.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.8.1.9 overexpression of wild-type and mutant enzymes from plasmids Escherichia coli
1.8.1.9 trxB gene, DNA sequence analysis Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
1.8.1.9 additional information transposon mutagenesis Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.8.1.9 32000
-
 2 * 32000, SDS-PAGE Escherichia coli
1.8.1.9 65800
-
 gel filtration Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
1.8.1.9 Escherichia coli
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.1.9 5,5'-dithiobis(2-nitrobenzoic acid) + NADPH i.e. DTNB Escherichia coli 2-nitro-5-thiobenzoate + NADP+
-
 ?
1.8.1.9 thioredoxin + NADP+ coupled assay with DTNB Escherichia coli thioredoxin disulfide + NADPH
-
 ?

Subunits

EC Number Subunits Comment Organism
1.8.1.9 dimer 2 * 32000, SDS-PAGE Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.1.9 NADPH
-
 Escherichia coli