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Literature summary extracted from
- Characterization of two active site mutations of thioredoxin reductase from Escherichia coli [published erratum appears in J Biol Chem 1989 Jul 15;264(20):12113] (1989), J. Biol. Chem., 264, 2656-2664.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.8.1.9 | overexpression of wild-type and mutant enzymes from plasmids | Escherichia coli |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 1.8.1.9 | C136S | site-directed mutagenesis, reduced activity | Escherichia coli |
| 1.8.1.9 | C139S | site-directed mutagenesis, reduced activity | Escherichia coli |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.8.1.9 | 0.00088 | - |
thioredoxin | mutant C136S | Escherichia coli |  |
| 1.8.1.9 | 0.00125 | - |
thioredoxin | wild-type | Escherichia coli | |
| 1.8.1.9 | 0.0017 | - |
thioredoxin | mutant C139S | Escherichia coli | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.8.1.9 | Escherichia coli | - |
enzyme with active site mutation | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.8.1.9 | wild-type from overexpressing cell line, mutants | Escherichia coli |
Reaction
| EC Number | Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|---|
| 1.8.1.9 | thioredoxin + NADP+ = thioredoxin disulfide + NADPH + H+ | catalytic mechanism | Escherichia coli |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.8.1.9 | thioredoxin + NADP+ | coupled assay with DTNB | Escherichia coli | thioredoxin disulfide + NADPH | - |
r | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.8.1.9 | TR | - |
Escherichia coli |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.8.1.9 | 2.38 | - |
thioredoxin | mutant C136S | Escherichia coli | |
| 1.8.1.9 | 13.2 | - |
thioredoxin | mutant C139S | Escherichia coli | |
| 1.8.1.9 | 22 | - |
thioredoxin | wild-type | Escherichia coli | |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.8.1.9 | FAD | - |
Escherichia coli | |
| 1.8.1.9 | NADPH | - |
Escherichia coli | |
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