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Literature summary extracted from

  • Gleason, F.K.; Lim, C.J.; Gerami-Nejad, M.; Fuchs, J.A.
    Characterization of Escherichia coli thioredoxins with altered active site residues (1990), Biochemistry, 29, 3701-3709.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.8.1.9 trxB gene, cloning of active site mutants, expression from plasmids Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.1.9 additional information
-
 additional information Km-values are pH-dependent Escherichia coli
1.8.1.9 0.002
-
 thioredoxin substrate wild-type, thioredoxin P34S, pH 8.0 Escherichia coli
1.8.1.9 0.0067
-
 thioredoxin K36R substrate mutant, pH 8.0 Escherichia coli
1.8.1.9 0.125
-
 thioredoxin-R substrate mutant, thioredoxin-CAC, pH 8.0 Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
1.8.1.9 Escherichia coli
-
 overproducing strain, trxB gene in a pUC vector
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.1.9 wild-type from overexpressing cell line, mutants Escherichia coli

Reaction

EC Number Reaction Comment Organism Reaction ID
1.8.1.9 thioredoxin + NADP+ = thioredoxin disulfide + NADPH + H+ oxidation-reduction cycle of thioredoxin Escherichia coli
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.8.1.9 additional information
-
 3 different assay methods Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.1.9 thioredoxin + NADP+
-
 Escherichia coli thioredoxin disulfide + NADPH
-
 ir
1.8.1.9 thioredoxin K36E + NADP+
-
 Escherichia coli thioredoxin K36E disulfide + NADPH + H+
-
 r
1.8.1.9 thioredoxin K36R + NADP+
-
 Escherichia coli thioredoxin K36R disulfide + NADPH + H+
-
 r
1.8.1.9 thioredoxin P34S + NADP+
-
 Escherichia coli thioredoxin P34S disulfide + NADPH + H+
-
 r
1.8.1.9 thioredoxin-CAC + NADP+
-
 Escherichia coli thioredoxin-CAC disulfide + NADPH + H+
-
 r
1.8.1.9 thioredoxin-R + NADP+
-
 Escherichia coli thioredoxin-R disulfide + NADPH + H+
-
 r

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.8.1.9 10.3
-
 thioredoxin-R mutant, pH 8.0 Escherichia coli
1.8.1.9 10.3
-
 thioredoxin-CAC mutant, pH 8.0 Escherichia coli
1.8.1.9 22.7
-
 thioredoxin K36E mutant, pH 8.0 Escherichia coli
1.8.1.9 22.7
-
 thioredoxin P34S mutant, pH 8.0 Escherichia coli
1.8.1.9 22.8
-
 thioredoxin wild-type, pH 8.0 Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.1.9 FAD
-
 Escherichia coli
1.8.1.9 NADPH
-
 Escherichia coli