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Literature summary extracted from
- Zinc fingers and thiol-disulfide oxidoreductase activities of chaperone DnaJ (2001), Biochemistry, 40, 14985-14994.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.8.4.2 | expression in Escherichia coli from plasmid | Escherichia coli |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.8.4.2 | EDTA | oxidation activity | Escherichia coli |  |
| 1.8.4.2 | guanidinium hydrochloride | destroys zinc finger thiols of DnaJ protein | Escherichia coli | |
| 1.8.4.2 | additional information | carboxylation of thiol groups abolish reduction activity | Escherichia coli |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.8.4.2 | Zn2+ | essential for activity | Escherichia coli | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.8.4.2 | 41000 | - |
2 * 41000, SDS-PAGE | Escherichia coli |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.8.4.2 | Escherichia coli | - |
thiol-disulfide oxidoreductase like activity from gene DnaJ | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.8.4.2 | recombinant enzyme | Escherichia coli |
Reaction
| EC Number | Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|---|
| 1.8.4.2 | 2 glutathione + protein-disulfide = glutathione-disulfide + protein-dithiol | C-X-X-C motif in active site for disulfide bonding, either oxidizing, reducing and isomerising activity | Escherichia coli | |
| 1.8.4.2 | 2 glutathione + protein-disulfide = glutathione-disulfide + protein-dithiol | zinc finger protein with several CXXC motifs | Escherichia coli |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.8.4.2 | additional information | - |
reductase activity after several treatments | Escherichia coli |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.8.4.2 | GSH + insulin | - |
Escherichia coli | GSSG + reduced insulin chain A and B | - |
? | |
| 1.8.4.2 | GSSG + reduced ribonuclease | - |
Escherichia coli | GSH + oxidized ribonuclease | - |
r | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.8.4.2 | dimer | 2 * 41000, SDS-PAGE | Escherichia coli |
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