BRENDA - Enzyme Database show

A method for measuring disulfide reduction by cultured mammalian cells: relative contributions of glutathione-dependent and glutathione-independent mechanisms

Biaglow, J.E.; Donahue, J.; Tuttle, S.; Held, K.; Chrestensen, C.; Mieyal, J.; Anal. Biochem. 281, 77-86 (2000)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.8.4.2
1,3-Bis(2-chloroethyl)-1-nitrosourea
-
Cricetulus griseus
1.8.4.2
1,3-Bis(2-chloroethyl)-1-nitrosourea
-
Homo sapiens
1.8.4.2
arsenite
-
Cricetulus griseus
1.8.4.2
arsenite
-
Homo sapiens
1.8.4.2
Phenylarsine oxide
-
Cricetulus griseus
1.8.4.2
Phenylarsine oxide
complete inhibition at 1 mM
Homo sapiens
1.8.4.2
selenium oxide
-
Homo sapiens
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.8.4.2
GSH + protein disulfide
Homo sapiens
-
GSSG + protein-dithiol
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.8.4.2
Cricetulus griseus
-
chinese hamster
-
1.8.4.2
Homo sapiens
-
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.8.4.2
insulinoma cell
-
Homo sapiens
-
1.8.4.2
lung
A549 carcinoma
Homo sapiens
-
1.8.4.2
mammary gland
MCF7 carcinoma
Homo sapiens
-
1.8.4.2
ovary
-
Cricetulus griseus
-
1.8.4.2
prostate gland
DU145 carcinoma
Homo sapiens
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
1.8.4.2
additional information
-
specific activities of different human carcinoma cell lines with different thiol and disulfide substrates
Homo sapiens
1.8.4.2
0.0035
-
A549 carcinoma cells, substrates GSH, cystamine or cystine
Homo sapiens
1.8.4.2
0.0075
-
A549 carcinoma cells, substrate dithiothreitol
Homo sapiens
1.8.4.2
0.03
-
A549 carcinoma cells, substrate hydroxyethyl disulfide
Homo sapiens
1.8.4.2
0.067
-
A549 carcinoma cells, substrate lipoate
Homo sapiens
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.8.4.2
alpha-lipoate + protein disulfide
-
394854
Homo sapiens
?
-
-
-
?
1.8.4.2
cysteamine + protein disulfide
low activity
394854
Homo sapiens
?
-
-
-
?
1.8.4.2
dithiothreitol + protein disulfide
low activity
394854
Homo sapiens
?
-
-
-
?
1.8.4.2
GSH + protein disulfide
-
394854
Homo sapiens
GSSG + protein-dithiol
-
-
-
?
1.8.4.2
hydroxyethyl disulfide + protein disulfide
-
394854
Homo sapiens
?
-
-
-
?
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.8.4.2
1,3-Bis(2-chloroethyl)-1-nitrosourea
-
Cricetulus griseus
1.8.4.2
1,3-Bis(2-chloroethyl)-1-nitrosourea
-
Homo sapiens
1.8.4.2
arsenite
-
Cricetulus griseus
1.8.4.2
arsenite
-
Homo sapiens
1.8.4.2
Phenylarsine oxide
-
Cricetulus griseus
1.8.4.2
Phenylarsine oxide
complete inhibition at 1 mM
Homo sapiens
1.8.4.2
selenium oxide
-
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.8.4.2
GSH + protein disulfide
Homo sapiens
-
GSSG + protein-dithiol
-
-
?
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.8.4.2
insulinoma cell
-
Homo sapiens
-
1.8.4.2
lung
A549 carcinoma
Homo sapiens
-
1.8.4.2
mammary gland
MCF7 carcinoma
Homo sapiens
-
1.8.4.2
ovary
-
Cricetulus griseus
-
1.8.4.2
prostate gland
DU145 carcinoma
Homo sapiens
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
1.8.4.2
additional information
-
specific activities of different human carcinoma cell lines with different thiol and disulfide substrates
Homo sapiens
1.8.4.2
0.0035
-
A549 carcinoma cells, substrates GSH, cystamine or cystine
Homo sapiens
1.8.4.2
0.0075
-
A549 carcinoma cells, substrate dithiothreitol
Homo sapiens
1.8.4.2
0.03
-
A549 carcinoma cells, substrate hydroxyethyl disulfide
Homo sapiens
1.8.4.2
0.067
-
A549 carcinoma cells, substrate lipoate
Homo sapiens
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.8.4.2
alpha-lipoate + protein disulfide
-
394854
Homo sapiens
?
-
-
-
?
1.8.4.2
cysteamine + protein disulfide
low activity
394854
Homo sapiens
?
-
-
-
?
1.8.4.2
dithiothreitol + protein disulfide
low activity
394854
Homo sapiens
?
-
-
-
?
1.8.4.2
GSH + protein disulfide
-
394854
Homo sapiens
GSSG + protein-dithiol
-
-
-
?
1.8.4.2
hydroxyethyl disulfide + protein disulfide
-
394854
Homo sapiens
?
-
-
-
?