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Literature summary extracted from

  • Pace, M.; Dixon, J.E.
    The nature of the multiple forms of bovine thiol:protein disulfide oxidoreductase (1979), Int. J. Pept. Protein Res., 14, 409-413.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.8.4.2 dimerization after prolonged storage at -20°C, freeze-thawing or heating at 60°C Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.8.4.2 60000
-
 1 * 60000, SDS-PAGE Bos taurus
1.8.4.2 60000
-
 2 * 60000, SDS-PAGE, dimerization after prolonged storage at -20°C, freeze-thawing or heating at 60°C, monomers held together by an intermolecular disulfide bond Bos taurus
1.8.4.2 120000
-
 MW 180000 also found in traces, monomer has MW 60000 Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
1.8.4.2 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.4.2
-
 Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.8.4.2 liver
-
 Bos taurus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.4.2 GSH + insulin
-
 Bos taurus GSSG + reduced insulin chain A and B
-
 ?
1.8.4.2 GSH + protein disulfide
-
 Bos taurus GSSG + protein-dithiol
-
 r

Subunits

EC Number Subunits Comment Organism
1.8.4.2 dimer 2 * 60000, SDS-PAGE, dimerization after prolonged storage at -20°C, freeze-thawing or heating at 60°C, monomers held together by an intermolecular disulfide bond Bos taurus
1.8.4.2 monomer 1 * 60000, SDS-PAGE Bos taurus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.8.4.2 37
-
 assay at Bos taurus