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Literature summary extracted from

  • Varandani, P.T.; Plumley, H.
    Mechanism of action of glutathione-insulin transhydrogenase. Presence of a functional sulfhydryl group for activity (1968), Biochim. Biophys. Acta, 151, 273-275.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.8.4.2 iodoacetamide treatment with thiol prior to incubation with GSH and substrate: no inhibition, preincubation with GSH and thiol reagent: inhibition Bos taurus
1.8.4.2 iodoacetate treatment with thiol prior to incubation with GSH and substrate: no inhibition, preincubation with GSH and thiol reagent: inhibition Bos taurus
1.8.4.2 N-ethylmaleimide treatment with thiol prior to incubation with GSH and substrate: no inhibition, preincubation with GSH and thiol reagent: inhibition Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.8.4.2 58000
-
 gel filtration Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
1.8.4.2 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.4.2
-
 Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.8.4.2 pancreas
-
 Bos taurus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.4.2 GSH + insulin
-
 Bos taurus GSSG + reduced insulin chain A and B
-
 ?
1.8.4.2 GSH + protein disulfide
-
 Bos taurus GSSG + protein-dithiol
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.8.4.2 37
-
 assay at Bos taurus