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Literature summary extracted from
- Rat-liver thiol: protein-disulphide oxidoreductase (1968), Biochim. Biophys. Acta, 167, 525-537.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.8.4.2 | EDTA | 5 mM: inhibition | Rattus norvegicus |  |
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 1.8.4.2 | EDTA 5mM, 37°C, phosphate buffer, total loss of activity within 30-60 min, can partially be prevented by NADPH | Rattus norvegicus |
| 1.8.4.2 | metal ions required for activity and maintenance of the proper conformation of the enzyme | Rattus norvegicus |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.8.4.2 | cysteine | at high concentration | Rattus norvegicus | |
| 1.8.4.2 | EDTA | 50% inhibition at 5 mM | Rattus norvegicus | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.8.4.2 | additional information | - |
additional information | - |
Rattus norvegicus | |
| 1.8.4.2 | 0.13 | - |
reduced dithiothreitol | - |
Rattus norvegicus | |
| 1.8.4.2 | 0.14 | - |
dihydrolipoamide | - |
Rattus norvegicus | |
| 1.8.4.2 | 0.2 | - |
2-mercaptoethanol | - |
Rattus norvegicus | |
| 1.8.4.2 | 0.2 | - |
dihydrolipoate | - |
Rattus norvegicus | |
| 1.8.4.2 | 0.27 | - |
cysteine | - |
Rattus norvegicus | |
| 1.8.4.2 | 0.31 | - |
GSH | - |
Rattus norvegicus | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.8.4.2 | Cs+ | 0.3 M: activates | Rattus norvegicus | |
| 1.8.4.2 | Mn2+ | 1 mM: activates | Rattus norvegicus | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.8.4.2 | Rattus norvegicus | - |
- |
- |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.8.4.2 | liver | - |
Rattus norvegicus | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.8.4.2 | 2-mercaptoethanol + protein disulfide | - |
Rattus norvegicus | 2-mercaptoethanol disulfide + protein-dithiol | - |
? | |
| 1.8.4.2 | dihydrolipoamide + protein disulfide | - |
Rattus norvegicus | ? | - |
? | |
| 1.8.4.2 | dihydrolipoate + protein disulfide | - |
Rattus norvegicus | ? | - |
? | |
| 1.8.4.2 | dithiothreitol + protein disulfide | - |
Rattus norvegicus | ? | - |
? | |
| 1.8.4.2 | GSH + protein disulfide | - |
Rattus norvegicus | GSSG + protein-dithiol | - |
? | |
| 1.8.4.2 | L-cysteine + protein disulfide | - |
Rattus norvegicus | cystine + protein-dithiol | - |
? | |
| 1.8.4.2 | reduced dithiothreitol + NADP+ | - |
Rattus norvegicus | oxidized dithiothreitol + NADPH + H+ | - |
r | |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.8.4.2 | 37 | - |
phosphate buffer, 5 h without loss of activity | Rattus norvegicus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.8.4.2 | 7.5 | - |
GSH | Rattus norvegicus |
| 1.8.4.2 | 9 | - |
DL-dihydrolipoate | Rattus norvegicus |
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Release 2023.1 (January 2023)
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