EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.8.1.12 | additional information | activity is dependent on a redox-active cysteine at the active centre | Trypanosoma cruzi |
EC Number | Cloned (Comment) | Organism |
---|---|---|
1.8.1.12 | overexpression of wild-type and mutants C53S, C53A, C58S in Escherichia coli | Trypanosoma cruzi |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.8.1.12 | C53A | site-directed mutagenesis, without catalytic active cysteine residue in the active center, no activity with trypanothione disulfide and NADPH, but showing transhydrogenase activity between NADPH and thio-NADP+ | Trypanosoma cruzi |
1.8.1.12 | C53S | site-directed mutagenesis, without catalytic active cysteine residue in the active center, no activity with trypanothione disulfide and NADPH, but showing transhydrogenase activity between NADPH and thio-NADP+ | Trypanosoma cruzi |
1.8.1.12 | C58S | site-directed mutagenesis, without catalytic active cysteine residue in the active center, no activity with trypanothione disulfide and NADPH, but showing transhydrogenase activity between NADPH and thio-NADP+ | Trypanosoma cruzi |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.8.1.12 | 0.004 | 0.015 | thio-NADP+ | recombinant mutants C53S, C53A, C58S | Trypanosoma cruzi | |
1.8.1.12 | 0.007 | 0.014 | NADPH | recombinant mutants C53S, C53A, C58S | Trypanosoma cruzi | |
1.8.1.12 | 0.017 | - |
NADPH | recombinant wild-type | Trypanosoma cruzi | |
1.8.1.12 | 0.024 | - |
trypanothione disulfide | recombinant wild-type | Trypanosoma cruzi | |
1.8.1.12 | 0.046 | - |
glutathionylspermidine disulfide | recombinant wild-type | Trypanosoma cruzi |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.8.1.12 | Trypanosoma cruzi | - |
Silvio strain | - |
1.8.1.12 | Trypanosoma cruzi Silvio | - |
Silvio strain | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.8.1.12 | recombinant wild-type and mutants C53S, C53A, C58S from Escherichia coli | Trypanosoma cruzi |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.8.1.12 | 0.18 | - |
recombinant mutant C58S: transhydrogenase activity between NADPH and thio-NADP+ | Trypanosoma cruzi |
1.8.1.12 | 0.21 | - |
recombinant mutant C53A: transhydrogenase activity between NADPH and thio-NADP+ | Trypanosoma cruzi |
1.8.1.12 | 0.44 | - |
recombinant mutant C53S: transhydrogenase activity between NADPH and thio-NADP+ | Trypanosoma cruzi |
1.8.1.12 | 6.49 | - |
recombinant wild-type: transhydrogenase activity between NADPH and thio-NADP+ | Trypanosoma cruzi |
1.8.1.12 | 143.2 | - |
purified recombinant enzyme | Trypanosoma cruzi |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.8.1.12 | glutathionylspermidine disulfide + NADPH | - |
Trypanosoma cruzi | 2-glutathionylspermidine + NADP+ | - |
? | |
1.8.1.12 | glutathionylspermidine disulfide + NADPH | - |
Trypanosoma cruzi Silvio | 2-glutathionylspermidine + NADP+ | - |
? | |
1.8.1.12 | additional information | wild-type enzyme shows transhydrogenase activity between NADPH and thio-NADP+ | Trypanosoma cruzi | ? | - |
? | |
1.8.1.12 | additional information | recombinant mutants C53S, C53A, C58S: no activity with trypanothione disulfide and NADPH, but showing transhydrogenase activity between NADPH and thio-NADP+ | Trypanosoma cruzi | ? | - |
? | |
1.8.1.12 | additional information | wild-type enzyme shows transhydrogenase activity between NADPH and thio-NADP+ | Trypanosoma cruzi Silvio | ? | - |
? | |
1.8.1.12 | additional information | recombinant mutants C53S, C53A, C58S: no activity with trypanothione disulfide and NADPH, but showing transhydrogenase activity between NADPH and thio-NADP+ | Trypanosoma cruzi Silvio | ? | - |
? | |
1.8.1.12 | NADPH + H+ + thio-NADP+ | - |
Trypanosoma cruzi | NADP+ + thio-NADPH + H+ | - |
r | |
1.8.1.12 | NADPH + H+ + thio-NADP+ | - |
Trypanosoma cruzi Silvio | NADP+ + thio-NADPH + H+ | - |
r | |
1.8.1.12 | trypanothione disulfide + NADPH | - |
Trypanosoma cruzi | trypanothione + NADP+ | - |
? | |
1.8.1.12 | trypanothione disulfide + NADPH | - |
Trypanosoma cruzi Silvio | trypanothione + NADP+ | - |
? | |
1.8.1.12 | trypanothione disulfide + thio-NADPH + H+ | - |
Trypanosoma cruzi | trypanothione + thio-NADP+ | - |
r |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.8.1.12 | TR | - |
Trypanosoma cruzi |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.8.1.12 | 25 | - |
assay at | Trypanosoma cruzi |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.8.1.12 | 0.19 | 0.47 | thio-NADPH | recombinant mutants C53S, C53A, C58S | Trypanosoma cruzi | |
1.8.1.12 | 0.21 | 0.51 | NADPH | recombinant mutants C53S, C53A, C58S | Trypanosoma cruzi | |
1.8.1.12 | 6.33 | - |
NADPH | recombinant wild-type | Trypanosoma cruzi | |
1.8.1.12 | 7.02 | - |
thio-NADPH | recombinant wild-type | Trypanosoma cruzi | |
1.8.1.12 | 85.4 | - |
glutathionylspermidine disulfide | recombinant wild-type | Trypanosoma cruzi | |
1.8.1.12 | 108 | - |
trypanothione disulfide | recombinant wild-type | Trypanosoma cruzi |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.8.1.12 | beta-thio-NADPH | - |
Trypanosoma cruzi | |
1.8.1.12 | FAD | - |
Trypanosoma cruzi | |
1.8.1.12 | NADPH | - |
Trypanosoma cruzi |