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Literature summary extracted from

  • Zheng, R.; Cenas, N.; Blanchard, J.S.
    Catalytic and potentiometric characterization of E201D and E201Q mutants of Trypanosoma congolense trypanothione reductase (1995), Biochemistry, 34, 12697-12703.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.8.1.12 additional information activity is dependent on a redox-active cysteine at the active centre Trypanosoma congolense

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.8.1.12 expression of wild-type and mutant enzymes in Escherichia coli Trypanosoma congolense

Protein Variants

EC Number Protein Variants Comment Organism
1.8.1.12 E201D by site-directed mutagenesis, 5% reductive activity and 50fold increased oxidative activity both compared to wild-type, enhanced quinone reductase activity Trypanosoma congolense
1.8.1.12 E201Q by site-directed mutagenesis, 5% reductive activity and 50fold increased oxidative activity both compared to wild-type, enhanced quinone reductase activity Trypanosoma congolense

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.8.1.12 3-acetylpyridine adenine nucleotide
-
 Trypanosoma congolense
1.8.1.12 NADP+
-
 Trypanosoma congolense
1.8.1.12 oxidized 3-acetylpyridine adenine nucleotide wild-type and mutant E201D and E201Q Trypanosoma congolense
1.8.1.12 thio-NADP+ wild-type and mutant E201D and E201Q Trypanosoma congolense

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.1.12 0.0012
-
 NADPH
-
 Trypanosoma congolense
1.8.1.12 0.0015 0.002 NADPH mutant E201D and E201Q Trypanosoma congolense
1.8.1.12 0.0023 0.0066 beta-thio-NADPH mutant E201D and E201Q Trypanosoma congolense
1.8.1.12 0.003
-
 3-acetylpyridine adenine dinucleotide mutant E201D and E201Q Trypanosoma congolense
1.8.1.12 0.0039
-
 3-acetylpyridine adenine dinucleotide
-
 Trypanosoma congolense
1.8.1.12 0.0113
-
 beta-thio-NADPH
-
 Trypanosoma congolense

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.8.1.12 trypanothione disulfide + NADPH Trypanosoma congolense physiological function is to protect the organism from oxidative stress and to assist in maintaining low intracellular levels of hydrogen peroxide trypanothione + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.8.1.12 Trypanosoma congolense
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.1.12 wild-type and mutants recombinant from Escherichia coli Trypanosoma congolense

Reaction

EC Number Reaction Comment Organism Reaction ID
1.8.1.12 trypanothione + NADP+ = trypanothione disulfide + NADPH + H+ hydride ion transfer reaction of the pro-S-hydrogen of NADPH to N5 of FAD, redox potentials Trypanosoma congolense
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.1.12 1,4-benzoquinone + NADPH + H+
-
 Trypanosoma congolense ?
-
 ?
1.8.1.12 9,10-phenanthrenequinone + NADPH + H+
-
 Trypanosoma congolense ?
-
 ?
1.8.1.12 trypanothione disulfide + beta-thio-NADPH wild-type and mutant enzymes Trypanosoma congolense trypanothione + beta-thio-NADP+
-
 ?
1.8.1.12 trypanothione disulfide + NADPH
-
 Trypanosoma congolense trypanothione + NADP+
-
 ir
1.8.1.12 trypanothione disulfide + NADPH physiological function is to protect the organism from oxidative stress and to assist in maintaining low intracellular levels of hydrogen peroxide Trypanosoma congolense trypanothione + NADP+
-
 ?
1.8.1.12 trypanothione disulfide + reduced 3-acetylpyridine adenine dinucleotide wild-type and mutant enzymes Trypanosoma congolense trypanothione + oxidized 3-acetylpyridine adenine dinucleotide
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.8.1.12 TR
-
 Trypanosoma congolense

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.8.1.12 2.9
-
 9,10-phenanthrenequinone
-
 Trypanosoma congolense
1.8.1.12 4.8
-
 1,4-benzoquinone
-
 Trypanosoma congolense
1.8.1.12 5.1
-
 trypanothione disulfide mutant E201Q Trypanosoma congolense
1.8.1.12 5.3
-
 trypanothione disulfide mutant E201D Trypanosoma congolense
1.8.1.12 9.4
-
 9,10-phenanthrenequinone mutant E201Q Trypanosoma congolense
1.8.1.12 13.6
-
 1,4-benzoquinone mutant E201D Trypanosoma congolense
1.8.1.12 24.5
-
 1,4-benzoquinone mutant E201Q Trypanosoma congolense
1.8.1.12 29
-
 9,10-phenanthrenequinone mutant E201D Trypanosoma congolense
1.8.1.12 122
-
 trypanothione disulfide
-
 Trypanosoma congolense

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.1.12 3-acetylpyridine adenine dinucleotide
-
 Trypanosoma congolense
1.8.1.12 beta-thio-NADPH
-
 Trypanosoma congolense
1.8.1.12 FAD
-
 Trypanosoma congolense
1.8.1.12 NADPH
-
 Trypanosoma congolense

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.8.1.12 0.0017
-
 3-acetylpyridine adenine nucleotide mutant E201Q Trypanosoma congolense
1.8.1.12 0.0033
-
 3-acetylpyridine adenine nucleotide mutant E201D Trypanosoma congolense
1.8.1.12 0.0054
-
 thio-NADP+ mutant E201D, slopes Trypanosoma congolense
1.8.1.12 0.0084
-
 3-acetylpyridine adenine nucleotide
-
 Trypanosoma congolense
1.8.1.12 0.011
-
 NADP+ wild-type and mutant E201D Trypanosoma congolense
1.8.1.12 0.011
-
 thio-NADP+ mutant E201Q Trypanosoma congolense
1.8.1.12 0.014
-
 NADP+ mutant E201Q Trypanosoma congolense
1.8.1.12 0.051
-
 thio-NADP+ mutant E201D, intercepts Trypanosoma congolense