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Literature summary extracted from

  • Lascano, H.R.; Casano, L.M.; Melchiorre, M.N.; Trippi, V.S.
    Biochemical and molecular characterisation of wheat chloroplastic glutathione reductase (2001), Biol. Plant., 44, 509-516.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.8.1.7 2 isoforms: DNA sequence analysis, multiple gene copies Triticum aestivum

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.8.1.7 diethyl dicarbonate chloroplast enzyme, 100% inhibition at 4 mM, 23% inhibition at 1 mM Triticum aestivum
1.8.1.7 N-ethylmaleimide chloroplast enzyme, 90% inhibition at 1 mM, partially reversible by GSSG 0.5 mM Triticum aestivum
1.8.1.7 NADPH chloroplast enzyme, 50% inhibition at 0.1 mM Triticum aestivum
1.8.1.7 p-chloromercuribenzoate chloroplast enzyme, 96% inhibition at 1 mM Triticum aestivum
1.8.1.7 Phenylmethylsulfonylfluoride i.e. PMSF, chloroplast enzyme, slight inhibition Triticum aestivum
1.8.1.7 Zn2+ chloroplast enzyme, 94% inhibition at 0.5 mM, reversible and competitive to GSSG Triticum aestivum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.1.7 0.015
-
 NADPH chloroplast enzyme Triticum aestivum
1.8.1.7 0.04
-
 GSSG chloroplast enzyme Triticum aestivum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.8.1.7 chloroplast stroma Triticum aestivum 9507
-
1.8.1.7 chloroplast isoform dependent Triticum aestivum 9507
-
1.8.1.7 cytoplasm isoform dependent Triticum aestivum 5737
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.8.1.7 32000
-
 2 * 56000 + associated polypeptide 1 * 32000, SDS-PAGE Triticum aestivum
1.8.1.7 56000
-
 2 * 56000 + associated polypeptide 1 * 32000, SDS-PAGE Triticum aestivum
1.8.1.7 150000
-
 chloroplast enzyme, gel filtration Triticum aestivum

Organism

EC Number Organism UniProt Comment Textmining
1.8.1.7 Triticum aestivum
-
 2 charge- and mass-separable isoforms in chloroplasts and cytosol
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.1.7 chloroplast enzyme Triticum aestivum

Reaction

EC Number Reaction Comment Organism Reaction ID
1.8.1.7 2 glutathione + NADP+ = glutathione disulfide + NADPH + H+ sulfhydryl groups and histidine residues are essential for the catalytic properties of the enzyme Triticum aestivum
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.8.1.7 leaf
-
 Triticum aestivum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.8.1.7 96
-
 purified enzyme Triticum aestivum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.1.7 GSSG + NADPH highly specific for GSSG Triticum aestivum glutathione + NADP+
-
 r

Subunits

EC Number Subunits Comment Organism
1.8.1.7 oligomer 2 * 56000 + associated polypeptide 1 * 32000, SDS-PAGE Triticum aestivum

Synonyms

EC Number Synonyms Comment Organism
1.8.1.7 GR
-
 Triticum aestivum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.8.1.7 45
-
 exponential increase in activity up to 45°C Triticum aestivum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.8.1.7 8
-
 NADPH + GSSG Triticum aestivum