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Literature summary extracted from
- Purification of thioredoxin, thioredoxin reductase, and glutathione reductase by affinity chromatography (1977), J. Biol. Chem., 252, 6367-6372.
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.8.1.7 | 52000 | - |
2 * 52000, SDS-PAGE | Escherichia coli |
| 1.8.1.7 | 105000 | - |
gel filtration | Escherichia coli |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.8.1.7 | Escherichia coli | - |
strain 011 | - |
| 1.8.1.7 | Escherichia coli 11 | - |
strain 011 | - |
| 1.8.1.9 | Escherichia coli | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.8.1.7 | large scale | Escherichia coli |
| 1.8.1.9 | - |
Escherichia coli |
Reaction
| EC Number | Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|---|
| 1.8.1.9 | thioredoxin + NADP+ = thioredoxin disulfide + NADPH + H+ | oxidation-reduction cycle of thioredoxin | Escherichia coli |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.8.1.7 | 505 | - |
purified enzyme | Escherichia coli |
| 1.8.1.9 | 67 | - |
purified enzyme | Escherichia coli |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.8.1.7 | GSSG + NADPH | - |
Escherichia coli | glutathione + NADP+ | - |
? |  |
| 1.8.1.7 | GSSG + NADPH | - |
Escherichia coli 11 | glutathione + NADP+ | - |
? | |
| 1.8.1.9 | thioredoxin + NADP+ | - |
Escherichia coli | thioredoxin disulfide + NADPH | - |
ir | |
| 1.8.1.9 | thioredoxin + NADP+ | - |
Escherichia coli 11 | thioredoxin disulfide + NADPH | - |
ir | |
| 1.8.1.9 | thioredoxin + NADP+ | in presence of NADPH, coupled assay | Escherichia coli | thioredoxin disulfide + NADPH + H+ | - |
ir | |
| 1.8.1.9 | thioredoxin + NADP+ | i.e. DTNB | Escherichia coli | thioredoxin disulfide + NADPH + H+ | - |
ir | |
| 1.8.1.9 | thioredoxin + NADP+ | in presence of NADPH, coupled assay | Escherichia coli 11 | thioredoxin disulfide + NADPH + H+ | - |
ir | |
| 1.8.1.9 | thioredoxin + NADP+ | i.e. DTNB | Escherichia coli 11 | thioredoxin disulfide + NADPH + H+ | - |
ir | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.8.1.7 | dimer | 2 * 52000, SDS-PAGE | Escherichia coli |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.8.1.7 | FAD | - |
Escherichia coli | |
| 1.8.1.7 | NADPH | - |
Escherichia coli | |
| 1.8.1.9 | FAD | - |
Escherichia coli | |
| 1.8.1.9 | NADPH | - |
Escherichia coli | |
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Release 2023.1 (January 2023)
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