BRENDA - Enzyme Database show

Purification and properties of glutathione reductase from the cyanobacterium Anabaena sp. strain 7119

Serrano, A.; Rivas, J.; Losada, M.; J. Bacteriol. 158, 317-324 (1984)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.8.1.7
arsenite
inhibition increased by NADPH
Anabaena sp.
1.8.1.7
N-ethylmaleimide
-
Anabaena sp.
1.8.1.7
Zn2+
inhibition increased by NADPH
Anabaena sp.
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.8.1.7
0.009
-
NADPH
-
Anabaena sp.
1.8.1.7
0.21
-
GSSG
-
Anabaena sp.
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.8.1.7
54000
-
2 * 54000, SDS-PAGE, gel filtration in presence of urea
Anabaena sp.
1.8.1.7
104000
-
PAGE at different acrylamide concentrations, gel filtration
Anabaena sp.
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.8.1.7
Anabaena sp.
-
filamentous cyanobacterium
-
1.8.1.7
Anabaena sp. 7119
-
filamentous cyanobacterium
-
Purification (Commentary)
EC Number
Commentary
Organism
1.8.1.7
-
Anabaena sp.
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.8.1.7
249
-
purified enzyme
Anabaena sp.
Storage Stability
EC Number
Storage Stability
Organism
1.8.1.7
25°C, in the dark
Anabaena sp.
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.8.1.7
GSSG + NADPH
-
394741
Anabaena sp.
glutathione + NADP+
-
-
-
?
1.8.1.7
GSSG + NADPH
-
394741
Anabaena sp. 7119
glutathione + NADP+
-
-
-
?
1.8.1.7
additional information
branched mechanism
394741
Anabaena sp.
?
-
-
-
-
1.8.1.7
additional information
branched mechanism
394741
Anabaena sp. 7119
?
-
-
-
-
Subunits
EC Number
Subunits
Commentary
Organism
1.8.1.7
dimer
2 * 54000, SDS-PAGE, gel filtration in presence of urea
Anabaena sp.
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.8.1.7
9
-
-
Anabaena sp.
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.8.1.7
FAD
FAD enzyme
Anabaena sp.
1.8.1.7
NADPH
-
Anabaena sp.
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.8.1.7
FAD
FAD enzyme
Anabaena sp.
1.8.1.7
NADPH
-
Anabaena sp.
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.8.1.7
arsenite
inhibition increased by NADPH
Anabaena sp.
1.8.1.7
N-ethylmaleimide
-
Anabaena sp.
1.8.1.7
Zn2+
inhibition increased by NADPH
Anabaena sp.
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.8.1.7
0.009
-
NADPH
-
Anabaena sp.
1.8.1.7
0.21
-
GSSG
-
Anabaena sp.
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.8.1.7
54000
-
2 * 54000, SDS-PAGE, gel filtration in presence of urea
Anabaena sp.
1.8.1.7
104000
-
PAGE at different acrylamide concentrations, gel filtration
Anabaena sp.
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.8.1.7
-
Anabaena sp.
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.8.1.7
249
-
purified enzyme
Anabaena sp.
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
1.8.1.7
25°C, in the dark
Anabaena sp.
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.8.1.7
GSSG + NADPH
-
394741
Anabaena sp.
glutathione + NADP+
-
-
-
?
1.8.1.7
GSSG + NADPH
-
394741
Anabaena sp. 7119
glutathione + NADP+
-
-
-
?
1.8.1.7
additional information
branched mechanism
394741
Anabaena sp.
?
-
-
-
-
1.8.1.7
additional information
branched mechanism
394741
Anabaena sp. 7119
?
-
-
-
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.8.1.7
dimer
2 * 54000, SDS-PAGE, gel filtration in presence of urea
Anabaena sp.
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.8.1.7
9
-
-
Anabaena sp.