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Literature summary extracted from

  • Rendon, J.L.; Calcagno, M.; Mendoza-Hernandez, G.; Ondarza, R.N.
    Purification, properties, and oligomeric structure of glutathione reductase from the cyanobacterium Spirulina maxima (1986), Arch. Biochem. Biophys., 248, 215-223.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.8.1.7 repeated freezing and thawing, no effect Limnospira maxima
1.8.1.7 urea, activation from 0.4-0.6 M, inactivation at higher concentration Limnospira maxima

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.8.1.7 Urea activation: 0.4-0.6 M, inactivation at higher concentration Limnospira maxima

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.1.7 0.121
-
 GSSG
-
 Limnospira maxima
1.8.1.7 3.3
-
 mixed disulfide of CoA and glutathione
-
 Limnospira maxima

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.8.1.7 47000
-
 2 * 47000, SDS-PAGE, enzyme exists predominantly as a tetrameric species in equilibrium with a minor dimer fraction Limnospira maxima
1.8.1.7 47000
-
 4 * 47000, SDS-PAGE, enzyme exists predominantly as a tetrameric species in equilibrium with a minor dimer fraction Limnospira maxima
1.8.1.7 99800
-
 Spirulina maxima, pore gradient gel electrophoresis, dimer, enzyme exists predominantly as tetrameric species in equilibrium with a minor dimer fraction Limnospira maxima
1.8.1.7 177000
-
 sedimentation equilibrium, tetramer, enzyme exists predominantly as a tetrameric species in equilibrium with a minor dimer fraction Limnospira maxima

Organism

EC Number Organism UniProt Comment Textmining
1.8.1.7 Limnospira maxima
-
 cyanobacterium
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.1.7
-
 Limnospira maxima

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.8.1.7 238
-
-
 Limnospira maxima

Storage Stability

EC Number Storage Stability Organism
1.8.1.7 -20°C, 1 year Limnospira maxima

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.1.7 glutathione + NADP+ mixed disulfide of CoA and glutathione Limnospira maxima glutathione disulfide + NADPH + H+
-
 r
1.8.1.7 GSSG + NADPH mixed disulfide between coenzyme A and glutathione Limnospira maxima glutathione + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.8.1.7 dimer 2 * 47000, SDS-PAGE, enzyme exists predominantly as a tetrameric species in equilibrium with a minor dimer fraction Limnospira maxima
1.8.1.7 tetramer 4 * 47000, SDS-PAGE, enzyme exists predominantly as a tetrameric species in equilibrium with a minor dimer fraction Limnospira maxima

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.8.1.7 25
-
 assay at Limnospira maxima

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.8.1.7 55
-
 10 min, pH 7.0, stable Limnospira maxima

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.8.1.7 7
-
-
 Limnospira maxima

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.1.7 FAD FAD enzyme Limnospira maxima
1.8.1.7 flavin 1.06 mol of flavin per mol of subunit Limnospira maxima
1.8.1.7 NADPH absolute specificity for NADPH Limnospira maxima