BRENDA - Enzyme Database show

Purification and properties of a disulfide reductase obtained from Achromobacter starkeyi

Ruiz-Herrera, J.; Amezcua-Ortega, R.; Trujillo, A.; J. Biol. Chem. 243, 4083-4088 (1968)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.8.1.7
arsenite
-
Achromobacter starkeyi
1.8.1.7
Ca2+
-
Achromobacter starkeyi
1.8.1.7
Cu2+
-
Achromobacter starkeyi
1.8.1.7
Hg2+
-
Achromobacter starkeyi
1.8.1.7
iodoacetate
-
Achromobacter starkeyi
1.8.1.7
Mg2+
-
Achromobacter starkeyi
1.8.1.7
Mn2+
-
Achromobacter starkeyi
1.8.1.7
N-ethylmaleimide
slight
Achromobacter starkeyi
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.8.1.7
GSSG + NADPH
Achromobacter starkeyi
specific for GSSG
glutathione + NADP+
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.8.1.7
Achromobacter starkeyi
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.8.1.7
-
Achromobacter starkeyi
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.8.1.7
1.18
-
-
Achromobacter starkeyi
Storage Stability
EC Number
Storage Stability
Organism
1.8.1.7
-20°C, 2-3 months
Achromobacter starkeyi
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.8.1.7
GSSG + NADPH
5,5'-dithiobis(2-nitrobenzoic acid)
394713
Achromobacter starkeyi
glutathione + NADP+
-
-
-
?
1.8.1.7
GSSG + NADPH
specific for GSSG
394713
Achromobacter starkeyi
glutathione + NADP+
-
-
-
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.8.1.7
28
-
-
Achromobacter starkeyi
Temperature Range [°C]
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
1.8.1.7
15
40
15°C: about 25% of activity maximum, 40°C: about 50% of activity maximum
Achromobacter starkeyi
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.8.1.7
7
-
-
Achromobacter starkeyi
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.8.1.7
NADH
enzyme can utilize both NADH and NADPH, more active with NADPH
Achromobacter starkeyi
1.8.1.7
NADPH
can utilize both NADPH and NADH, more active with NADPH
Achromobacter starkeyi
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.8.1.7
NADH
enzyme can utilize both NADH and NADPH, more active with NADPH
Achromobacter starkeyi
1.8.1.7
NADPH
can utilize both NADPH and NADH, more active with NADPH
Achromobacter starkeyi
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.8.1.7
arsenite
-
Achromobacter starkeyi
1.8.1.7
Ca2+
-
Achromobacter starkeyi
1.8.1.7
Cu2+
-
Achromobacter starkeyi
1.8.1.7
Hg2+
-
Achromobacter starkeyi
1.8.1.7
iodoacetate
-
Achromobacter starkeyi
1.8.1.7
Mg2+
-
Achromobacter starkeyi
1.8.1.7
Mn2+
-
Achromobacter starkeyi
1.8.1.7
N-ethylmaleimide
slight
Achromobacter starkeyi
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.8.1.7
GSSG + NADPH
Achromobacter starkeyi
specific for GSSG
glutathione + NADP+
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.8.1.7
-
Achromobacter starkeyi
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.8.1.7
1.18
-
-
Achromobacter starkeyi
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
1.8.1.7
-20°C, 2-3 months
Achromobacter starkeyi
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.8.1.7
GSSG + NADPH
5,5'-dithiobis(2-nitrobenzoic acid)
394713
Achromobacter starkeyi
glutathione + NADP+
-
-
-
?
1.8.1.7
GSSG + NADPH
specific for GSSG
394713
Achromobacter starkeyi
glutathione + NADP+
-
-
-
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.8.1.7
28
-
-
Achromobacter starkeyi
Temperature Range [°C] (protein specific)
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
1.8.1.7
15
40
15°C: about 25% of activity maximum, 40°C: about 50% of activity maximum
Achromobacter starkeyi
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.8.1.7
7
-
-
Achromobacter starkeyi