BRENDA - Enzyme Database show

Asparagusate dehydrogenases and lipoyl dehydrogenase from Asparagus mitochondria. Physical, chemical, and enzymatic properties

Yanagawa, H.; Egami, F.; J. Biol. Chem. 251, 3637-3644 (1976)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
1.8.1.11
lecithin
drastic activation of lipoyl dehydrogenase activity, no activation of asparagusate dehydrogenase activity, enzyme II
Asparagus officinalis
1.8.1.11
NAD+
reduction of lipoic acid is activated
Asparagus officinalis
1.8.1.11
Tween 80
drastic activation of lipoyl dehydrogenase activity, no activation of asparagusate dehydrogenase activity, enzyme II
Asparagus officinalis
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.8.1.11
arsenite
low inhibition
Asparagus officinalis
1.8.1.11
asparagusic acid
inhibits lipoyl dehydrogenase activity
Asparagus officinalis
1.8.1.11
cetyltrimethyl ammonium bromide
affects lipoyl dehydrogenase activity
Asparagus officinalis
1.8.1.11
FAD
strongly inhibits the two reductase activities
Asparagus officinalis
1.8.1.11
Hg2+
inhibitory
Asparagus officinalis
1.8.1.11
iodoacetamide
low inhibition
Asparagus officinalis
1.8.1.11
additional information
inhibitors indicate an involvement of protein disulfide linkage or thiol group in the catalytic site
Asparagus officinalis
1.8.1.11
N-ethylmaleimide
-
Asparagus officinalis
1.8.1.11
NADH
high concentrations, increase of inhibitory effect by addition of arsenite
Asparagus officinalis
1.8.1.11
p-chloromercuribenzoic acid
-
Asparagus officinalis
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.8.1.11
0.8
-
K3Fe(CN)6
enzyme II
Asparagus officinalis
1.8.1.11
0.9
-
K3Fe(CN)6
enzyme I
Asparagus officinalis
1.8.1.11
3
3.3
lipoate
enzyme I and II
Asparagus officinalis
1.8.1.11
3
-
lipoic acid
-
Asparagus officinalis
1.8.1.11
20
21.5
Asparagusate
enzyme I and II
Asparagus officinalis
1.8.1.11
20
-
asparagusic acid
-
Asparagus officinalis
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.8.1.11
mitochondrion
-
Asparagus officinalis
5739
-
1.8.1.11
soluble
-
Asparagus officinalis
-
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.8.1.11
110000
111000
ultracentrifugation
Asparagus officinalis
1.8.1.11
112000
-
calculation of amino acid content; enzyme I and II, gel filtration
Asparagus officinalis
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.8.1.11
asparagusate + NADH
Asparagus officinalis
-
dihydroasparagusate + NAD+
-
Asparagus officinalis
r
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.8.1.11
Asparagus officinalis
-
2 asparagusate dehydrogenases with lipoyl dehydrogenase activity
-
Purification (Commentary)
EC Number
Commentary
Organism
1.8.1.11
gel filtration, ion-exchange, ultracentrifugation, copurification of type I and II
Asparagus officinalis
Reaction
EC Number
Reaction
Commentary
Organism
1.8.1.11
3-mercapto-2-mercaptomethylpropanoate + NAD+ = asparagusate + NADH + H+
exhibits diaphorase activity; exhibits lipoyl dehydrogenase activity
Asparagus officinalis
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.8.1.11
shoot
-
Asparagus officinalis
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.8.1.11
0.215
-
enzyme/fraction II towards asparagusic acid
Asparagus officinalis
1.8.1.11
0.425
-
enzyme/fraction I towards asparagusic acid
Asparagus officinalis
1.8.1.11
0.5464
-
enzyme/fraction II towards lipoic acid
Asparagus officinalis
1.8.1.11
1.082
-
enzyme/fraction I towards lipoic acid
Asparagus officinalis
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.8.1.11
2 ferricyanide + NADH
-
394703
Asparagus officinalis
2 ferrocyanide + NAD+ + H+
-
394703
Asparagus officinalis
?
1.8.1.11
asparagusate + NADH
-
394703
Asparagus officinalis
dihydroasparagusate + NAD+
-
394703
Asparagus officinalis
r
1.8.1.11
asparagusic acid + NADH
reverse reaction very slow
394703
Asparagus officinalis
dihydroasparagusate + NAD+
-
394703
Asparagus officinalis
r
1.8.1.11
lipoic acid + NADH
reverse reaction very slow
394703
Asparagus officinalis
dihydrolipoic acid + NAD+
-
394703
Asparagus officinalis
r
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.8.1.11
25
-
assay at
Asparagus officinalis
1.8.1.11
40
45
-
Asparagus officinalis
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.8.1.11
50
-
5 min, activity completely retained; above, gradual loss of lipoyl dehydrogenase activity, complete loss at 90°C
Asparagus officinalis
1.8.1.11
60
70
5 min, 1.4-fold activation of asparagusate dehydrogenase activity
Asparagus officinalis
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.8.1.11
5.25
-
for K3Fe(CN)6 reduction
Asparagus officinalis
1.8.1.11
5.9
-
asparagusic and lipoic acid reduction
Asparagus officinalis
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.8.1.11
FAD
flavoprotein, approximately 1 mol of FAD per mol of protein
Asparagus officinalis
1.8.1.11
NAD+
up to 0.2 mM, stimulates the reduction activity if NADH concentration is 0.2 mM
Asparagus officinalis
1.8.1.11
NADH
0.1 mM optimal concentration for maximal activity; no replacement of NADH by NADPH
Asparagus officinalis
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
1.8.1.11
lecithin
drastic activation of lipoyl dehydrogenase activity, no activation of asparagusate dehydrogenase activity, enzyme II
Asparagus officinalis
1.8.1.11
NAD+
reduction of lipoic acid is activated
Asparagus officinalis
1.8.1.11
Tween 80
drastic activation of lipoyl dehydrogenase activity, no activation of asparagusate dehydrogenase activity, enzyme II
Asparagus officinalis
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.8.1.11
FAD
flavoprotein, approximately 1 mol of FAD per mol of protein
Asparagus officinalis
1.8.1.11
NAD+
up to 0.2 mM, stimulates the reduction activity if NADH concentration is 0.2 mM
Asparagus officinalis
1.8.1.11
NADH
0.1 mM optimal concentration for maximal activity; no replacement of NADH by NADPH
Asparagus officinalis
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.8.1.11
arsenite
low inhibition
Asparagus officinalis
1.8.1.11
asparagusic acid
inhibits lipoyl dehydrogenase activity
Asparagus officinalis
1.8.1.11
cetyltrimethyl ammonium bromide
affects lipoyl dehydrogenase activity
Asparagus officinalis
1.8.1.11
FAD
strongly inhibits the two reductase activities
Asparagus officinalis
1.8.1.11
Hg2+
inhibitory
Asparagus officinalis
1.8.1.11
iodoacetamide
low inhibition
Asparagus officinalis
1.8.1.11
additional information
inhibitors indicate an involvement of protein disulfide linkage or thiol group in the catalytic site
Asparagus officinalis
1.8.1.11
N-ethylmaleimide
-
Asparagus officinalis
1.8.1.11
NADH
high concentrations, increase of inhibitory effect by addition of arsenite
Asparagus officinalis
1.8.1.11
p-chloromercuribenzoic acid
-
Asparagus officinalis
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.8.1.11
0.8
-
K3Fe(CN)6
enzyme II
Asparagus officinalis
1.8.1.11
0.9
-
K3Fe(CN)6
enzyme I
Asparagus officinalis
1.8.1.11
3
3.3
lipoate
enzyme I and II
Asparagus officinalis
1.8.1.11
3
-
lipoic acid
-
Asparagus officinalis
1.8.1.11
20
21.5
Asparagusate
enzyme I and II
Asparagus officinalis
1.8.1.11
20
-
asparagusic acid
-
Asparagus officinalis
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.8.1.11
mitochondrion
-
Asparagus officinalis
5739
-
1.8.1.11
soluble
-
Asparagus officinalis
-
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.8.1.11
110000
111000
ultracentrifugation
Asparagus officinalis
1.8.1.11
112000
-
calculation of amino acid content; enzyme I and II, gel filtration
Asparagus officinalis
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.8.1.11
asparagusate + NADH
Asparagus officinalis
-
dihydroasparagusate + NAD+
-
Asparagus officinalis
r
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.8.1.11
gel filtration, ion-exchange, ultracentrifugation, copurification of type I and II
Asparagus officinalis
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.8.1.11
shoot
-
Asparagus officinalis
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.8.1.11
0.215
-
enzyme/fraction II towards asparagusic acid
Asparagus officinalis
1.8.1.11
0.425
-
enzyme/fraction I towards asparagusic acid
Asparagus officinalis
1.8.1.11
0.5464
-
enzyme/fraction II towards lipoic acid
Asparagus officinalis
1.8.1.11
1.082
-
enzyme/fraction I towards lipoic acid
Asparagus officinalis
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.8.1.11
2 ferricyanide + NADH
-
394703
Asparagus officinalis
2 ferrocyanide + NAD+ + H+
-
394703
Asparagus officinalis
?
1.8.1.11
asparagusate + NADH
-
394703
Asparagus officinalis
dihydroasparagusate + NAD+
-
394703
Asparagus officinalis
r
1.8.1.11
asparagusic acid + NADH
reverse reaction very slow
394703
Asparagus officinalis
dihydroasparagusate + NAD+
-
394703
Asparagus officinalis
r
1.8.1.11
lipoic acid + NADH
reverse reaction very slow
394703
Asparagus officinalis
dihydrolipoic acid + NAD+
-
394703
Asparagus officinalis
r
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.8.1.11
25
-
assay at
Asparagus officinalis
1.8.1.11
40
45
-
Asparagus officinalis
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.8.1.11
50
-
5 min, activity completely retained; above, gradual loss of lipoyl dehydrogenase activity, complete loss at 90°C
Asparagus officinalis
1.8.1.11
60
70
5 min, 1.4-fold activation of asparagusate dehydrogenase activity
Asparagus officinalis
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.8.1.11
5.25
-
for K3Fe(CN)6 reduction
Asparagus officinalis
1.8.1.11
5.9
-
asparagusic and lipoic acid reduction
Asparagus officinalis