Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Ma, M.; Zirngibl, C.; Linder, D.; Stetter, K.O.; Thauer, R.K.
    N5,N10-Methylenetetrahydromethanopterin dehydrogenase (H2-forming) from the extreme thermophile Methanopyrus kandleri (1991), Arch. Microbiol., 156, 43-48.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.12.98.2 0.002
-
 H+ pH 5.7 Methanopyrus kandleri
1.12.98.2 0.05
-
 N5,N10-methylenetetrahydromethanopterin
-
 Methanopyrus kandleri

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.12.98.2 KCl the enzyme requires the presence of salts, 50% of maximal activity is reached at 100 mM potassium phosphate, KCl or NaCl Methanopyrus kandleri
1.12.98.2 NaCl the enzyme requires the presence of salts, 50% of maximal activity is reached at 100 mM potassium phosphate, KCl or NaCl Methanopyrus kandleri
1.12.98.2 potassium phosphate the enzyme requires the presence of salts, 50% of maximal activity is reached at 100 mM potassium phosphate, KCl or NaCl Methanopyrus kandleri

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.12.98.2 44000
-
 4 * 44000, SDS-PAGE Methanopyrus kandleri
1.12.98.2 170000
-
 gel filtration Methanopyrus kandleri

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.12.98.2 additional information Methanopyrus kandleri the enzyme is involved in methanogenesis from CO2 and H2 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.12.98.2 Methanopyrus kandleri
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.12.98.2
-
 Methanopyrus kandleri

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.12.98.2 360
-
-
 Methanopyrus kandleri

Storage Stability

EC Number Storage Stability Organism
1.12.98.2 -20°C, under N2 gas phase, 20% loss of activity within 2 weeks Methanopyrus kandleri

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.12.98.2 additional information the enzyme is involved in methanogenesis from CO2 and H2 Methanopyrus kandleri ?
-
 ?
1.12.98.2 N5,N10-methylenetetrahydromethanopterin + H+ r Methanopyrus kandleri N5,N10-methenyltetrahydromethanopterin + H2
-
 ?
1.12.98.2 N5,N10-methylenetetrahydromethanopterin + H+ no reaction with: coenzyme F420, NAD+, NADP+ Methanopyrus kandleri N5,N10-methenyltetrahydromethanopterin + H2
-
 ?
1.12.98.2 N5,N10-methylenetetrahydromethanopterin + H+ reverse reaction is favoured under alkaline conditions Methanopyrus kandleri N5,N10-methenyltetrahydromethanopterin + H2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.12.98.2 tetramer 4 * 44000, SDS-PAGE Methanopyrus kandleri

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.12.98.2 additional information
-
-
 Methanopyrus kandleri

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.12.98.2 90
-
 60 min, stable, enzyme in cell extract Methanopyrus kandleri
1.12.98.2 90
-
 rapid inactivation of purified enzyme Methanopyrus kandleri

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.12.98.2 4.5 7 reaction rate increases hyperbolically with the proton concentration between pH 7 and pH 4.5 Methanopyrus kandleri