Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Renganathan, V.; Miki, K.; Gold, M.H.
    Multiple molecular forms of diarylpropane oxygenase, an H2O2-requiring, lignin-degrading enzyme from Phanerochaete chrysosporium (1985), Arch. Biochem. Biophys., 241, 304-314.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.11.1.14 0.032 0.049 H2O2
-
 Phanerodontia chrysosporium
1.11.1.14 0.055 0.095 veratryl alcohol
-
 Phanerodontia chrysosporium

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.11.1.14 extracellular
-
 Phanerodontia chrysosporium
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.11.1.14 Iron
-
 Phanerodontia chrysosporium

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.11.1.14 39000
-
 x * 39000, isozyme I, SDS-PAGE Phanerodontia chrysosporium
1.11.1.14 41000
-
 x * 41000, isozyme II, SDS-PAGE Phanerodontia chrysosporium
1.11.1.14 43000
-
 x * 43000, isozyme III, SDS-PAGE Phanerodontia chrysosporium

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.11.1.14 1-(3,4-diethoxyphenyl)-1,3-dihydroxy-2-(4-methoxyphenyl)-propane + O2 + H2O2 Phanerodontia chrysosporium
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.14 Phanerodontia chrysosporium
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.11.1.14 glycoprotein isozyme II: 6% w/w neutral carbohydrates Phanerodontia chrysosporium

Purification (Commentary)

EC Number Purification (Comment) Organism
1.11.1.14 3 isozymes by ion-exchange chromatography Phanerodontia chrysosporium

Reaction

EC Number Reaction Comment Organism Reaction ID
1.11.1.14 2 (3,4-dimethoxyphenyl)methanol + H2O2 = 2 (3,4-dimethoxyphenyl)methanol radical + 2 H2O mechanism Phanerodontia chrysosporium
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.11.1.14 culture supernatant
-
 Phanerodontia chrysosporium
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.11.1.14 8.48
-
 isozyme III Phanerodontia chrysosporium
1.11.1.14 11.8
-
 isozyme II Phanerodontia chrysosporium
1.11.1.14 18.75
-
 isozyme I Phanerodontia chrysosporium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.14 1-(3,4-diethoxyphenyl)-1,3-dihydroxy-2-(4-methoxy-phenyl)propane + O2 + H2O2
-
 Phanerodontia chrysosporium 1-(4'-methoxyphenyl)-1,2-dihydroxyethane + 3,4-diethoxybenzaldehyde
-
 ?
1.11.1.14 1-(3,4-diethoxyphenyl)-1,3-dihydroxy-2-(4-methoxyphenyl)-propane + O2 + H2O2
-
 Phanerodontia chrysosporium ?
-
 ?
1.11.1.14 1-(4-ethoxy-3-methoxyphenyl)-1,2-propene + O2 + H2O2 olefinic hydroxylation Phanerodontia chrysosporium 1-(4-ethoxy-3-methoxyphenyl)-1,2-dihydroxypropane
-
 ?
1.11.1.14 1-(4-ethoxy-3-methoxyphenyl)propane + O2 + H2O2
-
 Phanerodontia chrysosporium 1-(4-ethoxy-3-methoxyphenyl)1-hydroxypropane
-
 ?
1.11.1.14 4-methylthio-2-oxobutanoate + H2O2 only in presence of veratryl alcohol, it possibly reacts with a veratryl alcohol radical to produce ethylene Phanerodontia chrysosporium ?
-
 ?
1.11.1.14 additional information oxidation of various phenolic and non-phenolic lignin model-compounds Phanerodontia chrysosporium ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.11.1.14 ? x * 39000, isozyme I, SDS-PAGE Phanerodontia chrysosporium
1.11.1.14 ? x * 41000, isozyme II, SDS-PAGE Phanerodontia chrysosporium
1.11.1.14 ? x * 43000, isozyme III, SDS-PAGE Phanerodontia chrysosporium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.11.1.14 3
-
-
 Phanerodontia chrysosporium