EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.11.1.13 | H2O2 | H2O2-dependent | Phanerodontia chrysosporium | |
1.11.1.13 | additional information | 1 mM verartryl alcohol in growth medium increases activity 2fold | Phanerodontia chrysosporium |
EC Number | General Stability | Organism |
---|---|---|
1.11.1.13 | heat-inactivated protein, 100°C for 5 min, regains 80% of original activity by storage at 0°C for 1 h | Phanerodontia chrysosporium |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.11.1.13 | ascorbic acid | 0.1 mM, 100% inhibition | Phanerodontia chrysosporium | |
1.11.1.13 | Co2+ | 0.1 mM, 67% inhibition | Phanerodontia chrysosporium | |
1.11.1.13 | Cu2+ | 0.1 mM, 62% inhibition | Phanerodontia chrysosporium | |
1.11.1.13 | Fe2+ | 0.1 mM, 77% inhibition | Phanerodontia chrysosporium | |
1.11.1.13 | Fe3+ | 0.1 mM, 34% inhibition | Phanerodontia chrysosporium | |
1.11.1.13 | H2O2 | above 0.2 mM | Phanerodontia chrysosporium | |
1.11.1.13 | additional information | not inhibited by 5% ethanol, v/v; not inhibited by salicylic acid | Phanerodontia chrysosporium | |
1.11.1.13 | Superoxide dismutase | inhibits oxidation of vanillylacetone by about 80% | Phanerodontia chrysosporium |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.11.1.13 | extracellular | appears to be closely related to mycelium | Phanerodontia chrysosporium | - |
- |
1.11.1.14 | extracellular | - |
Phanerodontia chrysosporium | - |
- |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.11.1.13 | Mn2+ | stimulates | Phanerodontia chrysosporium | |
1.11.1.14 | Iron | hemoprotein containing protoporphyrin IX, high-spin ferri-heme-complex, 0.7 heme per enzyme molecule | Phanerodontia chrysosporium |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.11.1.13 | Mn2+ + H+ + H2O2 | Phanerodontia chrysosporium | involved in lignin-degradation | Mn3+ + H2O | the product Mn3+ is involved in the oxidative degradation of lignin in white-rot basidiomycetes, induced by veratryl alcohol | ? | |
1.11.1.14 | 1-(3,4-diethoxyphenyl)-1,3-dihydroxy-2-(4-methoxyphenyl)-propane + O2 + H2O2 | Phanerodontia chrysosporium | i.e. diarylpropane, involved in the oxidative breakdown of lignin in white rot basidiomycetes, induced by veratryl alcohol | ? | - |
? | |
1.11.1.14 | 1-(3,4-diethoxyphenyl)-1,3-dihydroxy-2-(4-methoxyphenyl)-propane + O2 + H2O2 | Phanerodontia chrysosporium BKM-F-1767 | i.e. diarylpropane, involved in the oxidative breakdown of lignin in white rot basidiomycetes, induced by veratryl alcohol | ? | - |
? | |
1.11.1.14 | 1-(3,4-diethoxyphenyl)-1,3-dihydroxy-2-(4-methoxyphenyl)-propane + O2 + H2O2 | Phanerodontia chrysosporium BKM-F 1767 | i.e. diarylpropane, involved in the oxidative breakdown of lignin in white rot basidiomycetes, induced by veratryl alcohol | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.11.1.13 | Phanerodontia chrysosporium | - |
- |
- |
1.11.1.13 | Phanerodontia chrysosporium | - |
white rot basidomycete | - |
1.11.1.13 | Phanerodontia chrysosporium BKM-F 1767 | - |
- |
- |
1.11.1.14 | Phanerodontia chrysosporium | - |
strains BKM-F-1767 | - |
1.11.1.14 | Phanerodontia chrysosporium BKM-F-1767 | - |
strains BKM-F-1767 | - |
EC Number | Posttranslational Modification | Comment | Organism |
---|---|---|---|
1.11.1.13 | glycoprotein | 17% carbohydrates | Phanerodontia chrysosporium |
1.11.1.14 | glycoprotein | 21% carbohydrate content | Phanerodontia chrysosporium |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.11.1.13 | 8.1-16fold purification | Phanerodontia chrysosporium |
1.11.1.14 | - |
Phanerodontia chrysosporium |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
1.11.1.13 | 2 Mn(II) + 2 H+ + H2O2 = 2 Mn(III) + 2 H2O | mechanism | Phanerodontia chrysosporium | |
1.11.1.13 | 2 Mn(II) + 2 H+ + H2O2 = 2 Mn(III) + 2 H2O | shows properties of a peroxidase and an oxidase | Phanerodontia chrysosporium | |
1.11.1.14 | 2 (3,4-dimethoxyphenyl)methanol + H2O2 = 2 (3,4-dimethoxyphenyl)methanol radical + 2 H2O | mechanism | Phanerodontia chrysosporium |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.11.1.14 | culture supernatant | - |
Phanerodontia chrysosporium | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.11.1.13 | 65 | 134 | depending on culture age | Phanerodontia chrysosporium |
1.11.1.14 | 12.5 | - |
- |
Phanerodontia chrysosporium |
EC Number | Storage Stability | Organism |
---|---|---|
1.11.1.13 | 5°C, unstable | Phanerodontia chrysosporium |
1.11.1.13 | frozen, tartrate buffer, pH 4.5, 6 months, stable | Phanerodontia chrysosporium |
1.11.1.14 | frozen, in crude concentrates of growth medium complete loss of activity within a month | Phanerodontia chrysosporium |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.11.1.13 | Mn2+ + H+ + H2O2 | completion of MnP catalytic cycle requires Mn2+ | Phanerodontia chrysosporium | Mn3+ + H2O | Mn3+ complex oxidizes a variety of organic substrates | ? | |
1.11.1.13 | Mn2+ + H+ + H2O2 | completion of MnP catalytic cycle requires Mn2+ | Phanerodontia chrysosporium | Mn3+ + H2O | Mn3+ oxidizes phenolic lignin model compounds | ? | |
1.11.1.13 | Mn2+ + H+ + H2O2 | completion of MnP catalytic cycle requires Mn2+ | Phanerodontia chrysosporium | Mn3+ + H2O | Mn3+ oxidizes vanillylacetone | ? | |
1.11.1.13 | Mn2+ + H+ + H2O2 | completion of MnP catalytic cycle requires Mn2+ | Phanerodontia chrysosporium | Mn3+ + H2O | Mn3+ oxidizes syringyl alcohol, syringyl aldehyde, syringic acid, syringaldazine, coniferyl alcohol, sinapic acid | ? | |
1.11.1.13 | Mn2+ + H+ + H2O2 | completion of MnP catalytic cycle requires Mn2+ | Phanerodontia chrysosporium | Mn3+ + H2O | Mn3+ oxidizes 2,6-dimethoxyphenol | ? | |
1.11.1.13 | Mn2+ + H+ + H2O2 | completion of MnP catalytic cycle requires Mn2+ | Phanerodontia chrysosporium | Mn3+ + H2O | Mn3+ oxidizes o-dianisidine | ? | |
1.11.1.13 | Mn2+ + H+ + H2O2 | completion of MnP catalytic cycle requires Mn2+ | Phanerodontia chrysosporium | Mn3+ + H2O | the diffusible product is Mn3+ | ? | |
1.11.1.13 | Mn2+ + H+ + H2O2 | completion of MnP catalytic cycle requires Mn2+ | Phanerodontia chrysosporium | Mn3+ + H2O | Mn3+ oxidizes a variety of phenols | ? | |
1.11.1.13 | Mn2+ + H+ + H2O2 | completion of MnP catalytic cycle requires Mn2+ | Phanerodontia chrysosporium | Mn3+ + H2O | Mn3+ oxidizes guaiacol | ? | |
1.11.1.13 | Mn2+ + H+ + H2O2 | oxidizes Mn2+ in presence of H2O2 to a higher oxidation state, enzyme activity is dependent on Mn2+ acting as electron carriers | Phanerodontia chrysosporium | Mn3+ + H2O | Mn3+ complex oxidizes a variety of organic substrates | ? | |
1.11.1.13 | Mn2+ + H+ + H2O2 | oxidizes Mn2+ in presence of H2O2 to a higher oxidation state, enzyme activity is dependent on Mn2+ acting as electron carriers | Phanerodontia chrysosporium | Mn3+ + H2O | Mn3+ oxidizes phenolic lignin model compounds | ? | |
1.11.1.13 | Mn2+ + H+ + H2O2 | oxidizes Mn2+ in presence of H2O2 to a higher oxidation state, enzyme activity is dependent on Mn2+ acting as electron carriers | Phanerodontia chrysosporium | Mn3+ + H2O | Mn3+ oxidizes vanillylacetone | ? | |
1.11.1.13 | Mn2+ + H+ + H2O2 | oxidizes Mn2+ in presence of H2O2 to a higher oxidation state, enzyme activity is dependent on Mn2+ acting as electron carriers | Phanerodontia chrysosporium | Mn3+ + H2O | Mn3+ oxidizes syringyl alcohol, syringyl aldehyde, syringic acid, syringaldazine, coniferyl alcohol, sinapic acid | ? | |
1.11.1.13 | Mn2+ + H+ + H2O2 | oxidizes Mn2+ in presence of H2O2 to a higher oxidation state, enzyme activity is dependent on Mn2+ acting as electron carriers | Phanerodontia chrysosporium | Mn3+ + H2O | Mn3+ oxidizes 2,6-dimethoxyphenol | ? | |
1.11.1.13 | Mn2+ + H+ + H2O2 | oxidizes Mn2+ in presence of H2O2 to a higher oxidation state, enzyme activity is dependent on Mn2+ acting as electron carriers | Phanerodontia chrysosporium | Mn3+ + H2O | Mn3+ oxidizes o-dianisidine | ? | |
1.11.1.13 | Mn2+ + H+ + H2O2 | oxidizes Mn2+ in presence of H2O2 to a higher oxidation state, enzyme activity is dependent on Mn2+ acting as electron carriers | Phanerodontia chrysosporium | Mn3+ + H2O | the diffusible product is Mn3+ | ? | |
1.11.1.13 | Mn2+ + H+ + H2O2 | oxidizes Mn2+ in presence of H2O2 to a higher oxidation state, enzyme activity is dependent on Mn2+ acting as electron carriers | Phanerodontia chrysosporium | Mn3+ + H2O | Mn3+ oxidizes a variety of phenols | ? | |
1.11.1.13 | Mn2+ + H+ + H2O2 | oxidizes Mn2+ in presence of H2O2 to a higher oxidation state, enzyme activity is dependent on Mn2+ acting as electron carriers | Phanerodontia chrysosporium | Mn3+ + H2O | Mn3+ oxidizes guaiacol | ? | |
1.11.1.13 | Mn2+ + H+ + H2O2 | involved in lignin-degradation | Phanerodontia chrysosporium | Mn3+ + H2O | the product Mn3+ is involved in the oxidative degradation of lignin in white-rot basidiomycetes, induced by veratryl alcohol | ? | |
1.11.1.13 | additional information | in absence of H2O2 the enzyme shows Mn-dependent oxidase activity against glutathione, dithiothreitol and dihydroxymaleic acid, forming H2O2 at the expense of oxygen | Phanerodontia chrysosporium | ? | - |
? | |
1.11.1.13 | additional information | in presence of H2O2 and Mn2+ the enzyme oxidizes a variety of phenolic compounds, especially vinyl and syringyl side-chain substituted substrates | Phanerodontia chrysosporium | ? | - |
? | |
1.11.1.13 | additional information | in absence of H2O2 the enzyme oxidizes Mn-dependently NADPH+ to NADP+ | Phanerodontia chrysosporium | ? | - |
? | |
1.11.1.14 | 1,2-bis(3,4-dimethoxyphenyl)propane-1,3-diol + H2O2 | - |
Phanerodontia chrysosporium | 3,4-dimethoxybenzaldehyde + 1-(3,4-dimethyl-phenyl)ethane-1,2-diol + H2O | - |
? | |
1.11.1.14 | 1,2-bis(3,4-dimethoxyphenyl)propane-1,3-diol + H2O2 | - |
Phanerodontia chrysosporium BKM-F-1767 | 3,4-dimethoxybenzaldehyde + 1-(3,4-dimethyl-phenyl)ethane-1,2-diol + H2O | - |
? | |
1.11.1.14 | 1,2-bis(3,4-dimethoxyphenyl)propane-1,3-diol + H2O2 | - |
Phanerodontia chrysosporium BKM-F 1767 | 3,4-dimethoxybenzaldehyde + 1-(3,4-dimethyl-phenyl)ethane-1,2-diol + H2O | - |
? | |
1.11.1.14 | 1-(3,4-diethoxyphenyl)-1,3-dihydroxy-2-(4-methoxyphenyl)-propane + O2 + H2O2 | i.e. diarylpropane, involved in the oxidative breakdown of lignin in white rot basidiomycetes, induced by veratryl alcohol | Phanerodontia chrysosporium | ? | - |
? | |
1.11.1.14 | 1-(3,4-diethoxyphenyl)-1,3-dihydroxy-2-(4-methoxyphenyl)-propane + O2 + H2O2 | i.e. diarylpropane, involved in the oxidative breakdown of lignin in white rot basidiomycetes, induced by veratryl alcohol | Phanerodontia chrysosporium BKM-F-1767 | ? | - |
? | |
1.11.1.14 | 1-(3,4-diethoxyphenyl)-1,3-dihydroxy-2-(4-methoxyphenyl)-propane + O2 + H2O2 | i.e. diarylpropane, involved in the oxidative breakdown of lignin in white rot basidiomycetes, induced by veratryl alcohol | Phanerodontia chrysosporium BKM-F 1767 | ? | - |
? | |
1.11.1.14 | 3,4-dimethoxybenzyl alcohol + H2O2 | veratryl alcohol | Phanerodontia chrysosporium | 3,4-dimethoxybenzaldehyde + H2O | - |
? | |
1.11.1.14 | 3,4-dimethoxybenzyl alcohol + H2O2 | veratryl alcohol | Phanerodontia chrysosporium BKM-F-1767 | 3,4-dimethoxybenzaldehyde + H2O | - |
? | |
1.11.1.14 | 3,4-dimethoxybenzyl alcohol + H2O2 | veratryl alcohol | Phanerodontia chrysosporium BKM-F 1767 | 3,4-dimethoxybenzaldehyde + H2O | - |
? | |
1.11.1.14 | additional information | oxidation of various phenolic and non-phenolic lignin model-compounds | Phanerodontia chrysosporium | ? | - |
? | |
1.11.1.14 | additional information | oxidation of various phenolic and non-phenolic lignin model-compounds | Phanerodontia chrysosporium BKM-F-1767 | ? | - |
? | |
1.11.1.14 | additional information | oxidation of various phenolic and non-phenolic lignin model-compounds | Phanerodontia chrysosporium BKM-F 1767 | ? | - |
? | |
1.11.1.14 | non-phenolic substrates + H2O2 | e.g. 1,2,4-trimethoxybenzene, 4,4'-dimethoxybiphenyl, isoeugenol methylether, 1-(3,4-dimethoxyphenyl)-2-(2, 4-dichlorophenoxyl)-ethanol, guaiacyl glycerolether | Phanerodontia chrysosporium | ? | - |
? | |
1.11.1.14 | non-phenolic substrates + H2O2 | e.g. 1,2,4-trimethoxybenzene, 4,4'-dimethoxybiphenyl, isoeugenol methylether, 1-(3,4-dimethoxyphenyl)-2-(2, 4-dichlorophenoxyl)-ethanol, guaiacyl glycerolether | Phanerodontia chrysosporium BKM-F-1767 | ? | - |
? | |
1.11.1.14 | non-phenolic substrates + H2O2 | e.g. 1,2,4-trimethoxybenzene, 4,4'-dimethoxybiphenyl, isoeugenol methylether, 1-(3,4-dimethoxyphenyl)-2-(2, 4-dichlorophenoxyl)-ethanol, guaiacyl glycerolether | Phanerodontia chrysosporium BKM-F 1767 | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.11.1.13 | ? | x * 45000-47000, SDS-PAGE | Phanerodontia chrysosporium |
1.11.1.14 | ? | x * 42000-43000, SDS-PAGE | Phanerodontia chrysosporium |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.11.1.13 | 30 | - |
assay at | Phanerodontia chrysosporium |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.11.1.13 | 100 | - |
complete inactivation after 5 min, 20% of original activity recovered after storage of heat-inactivated protein for 1 h at 0°C, no reactivation possible after boiling for 20 min | Phanerodontia chrysosporium |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.11.1.13 | heme | heme protein containing protoporphyrin IX, 0.7 heme per enzyme molecule, iron ions are coordinated with prosthetic groups as high-spin ferriheme complexes | Phanerodontia chrysosporium |