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Literature summary extracted from

  • Li, M.; Hubalek, F.; Newton-Vinson, P.; Edmondson, D.E.
    High-level expression of human liver monoamine oxidase A in Pichia pastoris: comparison with the enzyme expressed in Saccharomyces cerevisiae (2002), Protein Expr. Purif., 24, 152-162.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.4.3.4 advantage of the expression in Pichia pastoris compared with the expression in Saccharomyces cerevisiae: a higher level of homogeneity of the isolated enzyme Homo sapiens
1.4.3.4 expression in Pichia pastoris Homo sapiens
1.4.3.4 expression in Saccharomyces cerevisiae Homo sapiens
1.4.3.4 high-level expression in Pichia pastoris, higher expression level than in Saccharomyces cerevisiae and higher level of homogeneity of the isolated enzyme Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.3.4 Clorgyline
-
 Homo sapiens
1.4.3.4 N-(2-aminoethyl)-aryl-carboxamide
-
 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.3.4 additional information
-
 additional information comparison of Km of MAO expressed in Saccharomyces cerevisiae and Pichia pastoris Homo sapiens
1.4.3.4 0.89
-
 phenethylamine 11°C Homo sapiens
1.4.3.4 1.36
-
 dopamine 11°C Homo sapiens
1.4.3.4 2.92
-
 serotonin 11°C Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.3.4 60512
-
 x * 60512, electrospray mass spectroscopy, including one covalent FAD, MAO-A Homo sapiens
1.4.3.4 60512
-
 x * 60512, electrospray mass spectrometry Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
1.4.3.4 Homo sapiens
-
-
-
1.4.3.4 Homo sapiens P21397
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.3.4
-
 Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.4.3.4 liver
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.3.4 1.76
-
-
 Homo sapiens

Storage Stability

EC Number Storage Stability Organism
1.4.3.4 -20°C, in 50% glycerol v/v for up to 5 months without measurable loss of activity Homo sapiens
1.4.3.4 4°C, enzyme desalted by G-25 gel filtration, stable for 30 days Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.3.4 2-phenylethylamine + H2O + O2
-
 Homo sapiens 2-phenylethanal + NH3 + H2O2
-
 ?
1.4.3.4 dopamine + H2O + O2
-
 Homo sapiens (3,4-dihydroxyphenyl)acetaldehyde + NH3 + H2O2
-
 ?
1.4.3.4 kynuramine + H2O + O2
-
 Homo sapiens 3-(2-aminophenyl)-3-oxopropanal + NH3 + H2O2
-
 ?
1.4.3.4 RCH2NH2 + H2O + O2
-
 Homo sapiens RCHO + NH3 + H2O2
-
 ?
1.4.3.4 serotonin + H2O + O2
-
 Homo sapiens (5-hydroxy-1H-indol-3-yl)acetaldehyde + NH3 + H2O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.4.3.4 ? x * 60512, electrospray mass spectroscopy, including one covalent FAD, MAO-A Homo sapiens
1.4.3.4 ? x * 60512, electrospray mass spectrometry Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
1.4.3.4 MAO-A
-
 Homo sapiens
1.4.3.4 monoamine oxidase A
-
 Homo sapiens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.4.3.4 15
-
 below, enzyme expressed in Saccharomyces cerevisiae, 60 min stable Homo sapiens
1.4.3.4 20
-
 enzyme expressed in Pichia pastoris, 60 min stable Homo sapiens
1.4.3.4 20
-
 enzyme expressed in Saccharomyces cerevisiae, 60 min 15% loss of activity Homo sapiens
1.4.3.4 25
-
 enzyme expressed in Pichia pastoris, 60 min, 8% loss of activity Homo sapiens
1.4.3.4 25
-
 enzyme expressed in Saccharomyces cerevisiae, 60 min 50% loss of activity Homo sapiens
1.4.3.4 30
-
 enzyme expressed in Pichia pastoris, 60 min, 60% loss of activity Homo sapiens
1.4.3.4 30
-
 enzyme expressed in Saccharomyces cerevisiae, 60 min 80% loss of activity Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.4.3.4 0.249
-
 dopamine 11°C Homo sapiens
1.4.3.4 0.426
-
 serotonin 11°C Homo sapiens
1.4.3.4 1.481
-
 phenethylamine 11°C Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.3.4 8alpha-S-cysteinyl-FAD 1 mol per mol of enzyme Homo sapiens
1.4.3.4 flavin MAO-A contain 1 mol of 8-alpha-S-cysteinyl 406 FAD per mol Homo sapiens