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Literature summary extracted from

  • Berks, B.C.; Baratta, D.; Richardson, D.J.; Ferguson, S.J.
    Purification and characterization of a nitrous oxide reductase from Thiosphaera pantotropha. Implications for the mechanism of aerobic nitrous oxide reduction (1993), Eur. J. Biochem., 212, 467-476.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.7.2.4 an enzyme concentration of at least 0.01 mg per ml is necessary to avoid loss of activity due to dilution Paracoccus pantotrophus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.7.2.4 Acetylene
-
 Paracoccus pantotrophus
1.7.2.4 KCN
-
 Paracoccus pantotrophus
1.7.2.4 NaN3
-
 Paracoccus pantotrophus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.7.2.4 Cu wild-type 7.2 atoms per protein dimer Paracoccus pantotrophus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.7.2.4 66281
-
 2 * 66281, ESI mass spectrometry Paracoccus pantotrophus
1.7.2.4 73000
-
 2 * 73000, SDS-PAGE Paracoccus pantotrophus
1.7.2.4 115000
-
 gel filtration Paracoccus pantotrophus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.7.2.4 nitrous oxide + reduced acceptor Paracoccus pantotrophus
-
 nitrogen + H2O + acceptor
-
 ?
1.7.2.4 nitrous oxide + reduced acceptor Paracoccus pantotrophus LMD 82.5
-
 nitrogen + H2O + acceptor
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.7.2.4 Paracoccus pantotrophus
-
 LMD 82.5
-
1.7.2.4 Paracoccus pantotrophus
-
 formerly Thiosphaera pantotropha
-
1.7.2.4 Paracoccus pantotrophus LMD 82.5
-
 LMD 82.5
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.7.2.4 of anaerobically grown cells Paracoccus pantotrophus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.7.2.4 1
-
 ascorbate-reduced phenanzine methosulphate electrode assay Paracoccus pantotrophus
1.7.2.4 2.6
-
-
 Paracoccus pantotrophus
1.7.2.4 3.3
-
 photoreduced methyl viologen spectrophotometric assay Paracoccus pantotrophus
1.7.2.4 3.7
-
 dithionite-reduced methyl viologen electrode assay Paracoccus pantotrophus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.2.4 nitrous oxide + reduced acceptor
-
 Paracoccus pantotrophus nitrogen + H2O + acceptor
-
 ?
1.7.2.4 nitrous oxide + reduced acceptor dithionite-reduced methyl viologen as electron donor Paracoccus pantotrophus nitrogen + H2O + acceptor
-
 ?
1.7.2.4 nitrous oxide + reduced acceptor
-
 Paracoccus pantotrophus LMD 82.5 nitrogen + H2O + acceptor
-
 ?
1.7.2.4 nitrous oxide + reduced acceptor dithionite-reduced methyl viologen as electron donor Paracoccus pantotrophus LMD 82.5 nitrogen + H2O + acceptor
-
 ?

Subunits

EC Number Subunits Comment Organism
1.7.2.4 homodimer 2 * 73000, SDS-PAGE Paracoccus pantotrophus
1.7.2.4 homodimer 2 * 66281, ESI mass spectrometry Paracoccus pantotrophus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.7.2.4 8
-
 above pH 8 with reduced methyl viologen Paracoccus pantotrophus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.7.2.4 0.000025
-
 KCN
-
 Paracoccus pantotrophus
1.7.2.4 0.00064
-
 NaN3
-
 Paracoccus pantotrophus
1.7.2.4 0.035
-
 Acetylene
-
 Paracoccus pantotrophus