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Literature summary extracted from

  • Zucker, M.; Nason, A.
    A pyridine nucleotide-hydroxylamine reductase from Neurospora (1955), J. Biol. Chem., 213, 463-478.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.7.1.10 dialysis against 3 mM sodium diphosphate, 1 mM cysteine solution, pH 8, 2 h, less than 50% loss of activity, against 10 mM phosphate, pH 8, 1 h, complete loss of activity Neurospora crassa
1.7.1.10 little or no activity in phosphate buffer unless 1 mM EDTA is included in the reaction mixture Neurospora crassa

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.7.1.10 2,2'-dipyridyl
-
 Neurospora crassa
1.7.1.10 8-hydroxyquinoline
-
 Neurospora crassa
1.7.1.10 Cu2+
-
 Neurospora crassa
1.7.1.10 KCN 0.1 mM, complete inhibition Neurospora crassa
1.7.1.10 NaN3
-
 Neurospora crassa
1.7.1.10 o-phenanthroline
-
 Neurospora crassa
1.7.1.10 potassium ethyl xanthate
-
 Neurospora crassa
1.7.1.10 Salicylaldoxime
-
 Neurospora crassa
1.7.1.10 Sodium diethyl dithiocarbamate
-
 Neurospora crassa
1.7.1.10 sulfite
-
 Neurospora crassa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.7.1.10 0.07
-
 NADH
-
 Neurospora crassa
1.7.1.10 0.1
-
 NADPH
-
 Neurospora crassa
1.7.1.10 3.8
-
 hydroxylamine
-
 Neurospora crassa

Organism

EC Number Organism UniProt Comment Textmining
1.7.1.10 Neurospora crassa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.7.1.10 partial Neurospora crassa

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.7.1.10 additional information
-
 360.0 units/mg, 1 unit is defined as that amount of enzyme which causes a rate of change in extinction of 0.001 per minute Neurospora crassa

Storage Stability

EC Number Storage Stability Organism
1.7.1.10 -15°C, slow loss of activity over several weeks Neurospora crassa
1.7.1.10 4°C, overnight Neurospora crassa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.1.10 hydroxylamine + NADH no other substances beside nitrite and dinitrobenzene stimulate oxidation of NADPH Neurospora crassa NH3 + NAD+ + H2O
-
 ir
1.7.1.10 hydroxylamine + NADH no activity with O-methylhydroxylamine, hydrazine, nitrophenylhydroxylamine and salicylhydroxamic acid Neurospora crassa NH3 + NAD+ + H2O
-
 ir
1.7.1.10 hydroxylamine + NADPH + H+
-
 Neurospora crassa NH3 + NADP+ + H2O
-
 r
1.7.1.10 NO2- + NADH
-
 Neurospora crassa NH3 + H2O + NAD+
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.7.1.10 50
-
 5 min, stable Neurospora crassa

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.7.1.10 7.5 9.5 highest stability Neurospora crassa

Cofactor

EC Number Cofactor Comment Organism Structure
1.7.1.10 FAD stimulates Neurospora crassa
1.7.1.10 NADH
-
 Neurospora crassa
1.7.1.10 NADPH
-
 Neurospora crassa