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Literature summary extracted from

  • Roussos, G.G.; Nason, A.
    Pyridine nucleotide-nitrite and -hydroxylamine enzymes from soybean leaves (1960), J. Biol. Chem., 235, 2997-3007.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.7.1.10 2,2'-dipyridyl 1 mM, 84% inhibition Glycine max
1.7.1.10 8-hydroxyquinoline 1 mM, complete inhibition Glycine max
1.7.1.10 hydrazine 1 mM, complete inhibition Glycine max
1.7.1.10 KCN 0.1 mM, complete inhibition Glycine max
1.7.1.10 NaN3 1 mM, complete inhibition Glycine max
1.7.1.10 o-phenanthroline 1 mM, complete inhibition Glycine max
1.7.1.10 p-chloromercuribenzoate 0.1 mM, complete inhibition Glycine max
1.7.1.10 potassium ethyl xanthate
-
 Glycine max
1.7.1.10 Salicylaldoxime
-
 Glycine max
1.7.1.10 Sodium diethyl dithiocarbamate 1 mM, complete inhibition Glycine max

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.7.1.10 soluble
-
 Glycine max
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.7.1.10 Mn2+ 0.1 mM, approx. 6fold stimulation Glycine max

Organism

EC Number Organism UniProt Comment Textmining
1.7.1.10 Glycine max
-
 soybean
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.7.1.10 enzyme not seperated from pyridine nucleotide-nitrite reductase Glycine max

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.7.1.10 leaf
-
 Glycine max
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.7.1.10 additional information
-
 794.1 units/mg, 1 unit is defined as that amount of enzyme which causes a rate of change in extinction of 0.001 per minute Glycine max

Storage Stability

EC Number Storage Stability Organism
1.7.1.10 -20°C, 1 month, 60-90% loss of activity Glycine max
1.7.1.10 4°C, 1 week, no loss in activity Glycine max

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.1.10 hydroxylamine + NADH NADH is 1.5fold more effective than NADPH Glycine max NH3 + NAD+ + H2O
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.7.1.10 50
-
 complete loss of activity after 5 min Glycine max

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.7.1.10 6.6 7.1
-
 Glycine max

Cofactor

EC Number Cofactor Comment Organism Structure
1.7.1.10 additional information purified enzyme: absolute requirement for an unidentified, dissociable, heat-stable, organic factor obtained from soybean leaf extract, no flavin requirement Glycine max
1.7.1.10 NADH
-
 Glycine max
1.7.1.10 NADPH about 65% of the activity with NADH Glycine max