EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.7.7.1 | (NH4)2SO4 | slightly inhibitory | Spinacia oleracea | |
1.7.7.1 | cyanide | almost complete inhibition from 0.02 mM to 0.2 mM | Spinacia oleracea |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.7.7.1 | Iron | - |
Spinacia oleracea |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.7.7.1 | additional information | - |
the native enzyme with a MW of 85000 can be split into a modified form of a MW of 61000 that retains activity with the non-physiological electron-donor, methyl viologen but loses most of the ferredoxin-linked activity and an 24000 MW coupling protein fragment in which the ferredoxin-binding domain is located | Spinacia oleracea |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.7.7.1 | Spinacia oleracea | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.7.7.1 | - |
Spinacia oleracea |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.7.7.1 | 301.4 | - |
- |
Spinacia oleracea |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.7.7.1 | nitrite + reduced ferredoxin | - |
Spinacia oleracea | ammonia + oxidized ferredoxin | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.7.7.1 | More | spinach: the native enzyme with a molecular weight of 85000 can be split into a modified form with a MW of 61000 that retains activity with the non-physiological electron-donor methyl viologen, but loses most of the ferredoxin-linked activity and an 24000 MW coupling protein fragment in which the ferredoxin-binding domain is located | Spinacia oleracea |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.7.7.1 | siroheme | prosthetic group | Spinacia oleracea | |
1.7.7.1 | siroheme | 2 mol of siroheme per mol of enzyme | Spinacia oleracea |