EC Number | General Stability | Organism |
---|---|---|
1.7.7.1 | repetitive freezing and thawing or prolonged dialysis produces a loss of enzyme activity | Spinacia oleracea |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.7.7.1 | chloroplast | - |
Synechococcus elongatus PCC 7942 = FACHB-805 | 9507 | - |
1.7.7.1 | chloroplast | - |
Spinacia oleracea | 9507 | - |
1.7.7.1 | chloroplast | - |
[Chlorella] fusca | 9507 | - |
1.7.7.1 | chloroplast | - |
Cucurbita pepo | 9507 | - |
1.7.7.1 | chloroplast | - |
Anabaena cylindrica | 9507 | - |
1.7.7.1 | chloroplast | - |
Dunaliella tertiolecta | 9507 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.7.7.1 | Iron | - |
[Chlorella] fusca | |
1.7.7.1 | Iron | - |
Cucurbita pepo | |
1.7.7.1 | Iron | - |
Dunaliella tertiolecta | |
1.7.7.1 | Iron | enzyme contains 1 tetranuclear center (4Fe-4S) and 1 siroheme, minimum of 5 Fe atoms per active enzyme molecule | Spinacia oleracea |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.7.7.1 | nitrite + reduced ferredoxin | Synechococcus elongatus PCC 7942 = FACHB-805 | second enzyme of photosynthetic nitrate-reducing system | ammonia + oxidized ferredoxin | - |
? | |
1.7.7.1 | nitrite + reduced ferredoxin | Spinacia oleracea | second enzyme of photosynthetic nitrate-reducing system | ammonia + oxidized ferredoxin | - |
? | |
1.7.7.1 | nitrite + reduced ferredoxin | [Chlorella] fusca | second enzyme of photosynthetic nitrate-reducing system | ammonia + oxidized ferredoxin | - |
? | |
1.7.7.1 | nitrite + reduced ferredoxin | Cucurbita pepo | second enzyme of photosynthetic nitrate-reducing system | ammonia + oxidized ferredoxin | - |
? | |
1.7.7.1 | nitrite + reduced ferredoxin | Anabaena cylindrica | second enzyme of photosynthetic nitrate-reducing system | ammonia + oxidized ferredoxin | - |
? | |
1.7.7.1 | nitrite + reduced ferredoxin | Dunaliella tertiolecta | second enzyme of photosynthetic nitrate-reducing system | ammonia + oxidized ferredoxin | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.7.7.1 | Anabaena cylindrica | - |
- |
- |
1.7.7.1 | Cucurbita pepo | - |
- |
- |
1.7.7.1 | Dunaliella tertiolecta | - |
- |
- |
1.7.7.1 | Spinacia oleracea | - |
- |
- |
1.7.7.1 | Synechococcus elongatus PCC 7942 = FACHB-805 | - |
- |
- |
1.7.7.1 | [Chlorella] fusca | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.7.7.1 | homogeneity | Spinacia oleracea |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
1.7.7.1 | NH3 + 2 H2O + 3 oxidized ferredoxin = nitrite + 3 reduced ferredoxin + 7 H+ | mechanism | Synechococcus elongatus PCC 7942 = FACHB-805 | |
1.7.7.1 | NH3 + 2 H2O + 3 oxidized ferredoxin = nitrite + 3 reduced ferredoxin + 7 H+ | mechanism | Spinacia oleracea | |
1.7.7.1 | NH3 + 2 H2O + 3 oxidized ferredoxin = nitrite + 3 reduced ferredoxin + 7 H+ | mechanism | [Chlorella] fusca | |
1.7.7.1 | NH3 + 2 H2O + 3 oxidized ferredoxin = nitrite + 3 reduced ferredoxin + 7 H+ | mechanism | Cucurbita pepo | |
1.7.7.1 | NH3 + 2 H2O + 3 oxidized ferredoxin = nitrite + 3 reduced ferredoxin + 7 H+ | mechanism | Anabaena cylindrica | |
1.7.7.1 | NH3 + 2 H2O + 3 oxidized ferredoxin = nitrite + 3 reduced ferredoxin + 7 H+ | mechanism | Dunaliella tertiolecta |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.7.7.1 | leaf | - |
Spinacia oleracea | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.7.7.1 | 108 | - |
- |
Spinacia oleracea |
EC Number | Storage Stability | Organism |
---|---|---|
1.7.7.1 | -20°C, 50% glycerol, for at least 6 months | Spinacia oleracea |
1.7.7.1 | 4°C, 0.1 M potassium phosphate buffer, pH 7.7, 1 week | Spinacia oleracea |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.7.7.1 | nitrite + reduced ferredoxin | - |
Synechococcus elongatus PCC 7942 = FACHB-805 | ammonia + oxidized ferredoxin | - |
? | |
1.7.7.1 | nitrite + reduced ferredoxin | - |
Spinacia oleracea | ammonia + oxidized ferredoxin | - |
? | |
1.7.7.1 | nitrite + reduced ferredoxin | - |
[Chlorella] fusca | ammonia + oxidized ferredoxin | - |
? | |
1.7.7.1 | nitrite + reduced ferredoxin | - |
Cucurbita pepo | ammonia + oxidized ferredoxin | - |
? | |
1.7.7.1 | nitrite + reduced ferredoxin | - |
Anabaena cylindrica | ammonia + oxidized ferredoxin | - |
? | |
1.7.7.1 | nitrite + reduced ferredoxin | - |
Dunaliella tertiolecta | ammonia + oxidized ferredoxin | - |
? | |
1.7.7.1 | nitrite + reduced ferredoxin | second enzyme of photosynthetic nitrate-reducing system | Synechococcus elongatus PCC 7942 = FACHB-805 | ammonia + oxidized ferredoxin | - |
? | |
1.7.7.1 | nitrite + reduced ferredoxin | second enzyme of photosynthetic nitrate-reducing system | Spinacia oleracea | ammonia + oxidized ferredoxin | - |
? | |
1.7.7.1 | nitrite + reduced ferredoxin | second enzyme of photosynthetic nitrate-reducing system | [Chlorella] fusca | ammonia + oxidized ferredoxin | - |
? | |
1.7.7.1 | nitrite + reduced ferredoxin | second enzyme of photosynthetic nitrate-reducing system | Cucurbita pepo | ammonia + oxidized ferredoxin | - |
? | |
1.7.7.1 | nitrite + reduced ferredoxin | second enzyme of photosynthetic nitrate-reducing system | Anabaena cylindrica | ammonia + oxidized ferredoxin | - |
? | |
1.7.7.1 | nitrite + reduced ferredoxin | second enzyme of photosynthetic nitrate-reducing system | Dunaliella tertiolecta | ammonia + oxidized ferredoxin | - |
? |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.7.7.1 | 40 | - |
stable | Spinacia oleracea |
1.7.7.1 | 60 | - |
10 min, more than 95% loss of activity | Spinacia oleracea |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.7.7.1 | 7 | 8 | - |
Spinacia oleracea |
1.7.7.1 | 7 | 8 | - |
[Chlorella] fusca |
1.7.7.1 | 7 | 8 | - |
Cucurbita pepo |
1.7.7.1 | 7 | 8 | - |
Anabaena cylindrica |
1.7.7.1 | 7 | 8 | - |
Dunaliella tertiolecta |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.7.7.1 | siroheme | prosthetic group | Spinacia oleracea | |
1.7.7.1 | siroheme | 1 mol siroheme per mol of enzyme | Spinacia oleracea |