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Literature summary extracted from

  • Romero, L.C.; Galvan, F.; Vega, J.M.
    Purification and properties of the siroheme-containing ferredoxin-nitrite reductase from Chlamydomonas reinhardtii (1987), Biochim. Biophys. Acta, 914, 55-63.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.7.7.1 CO
-
 Chlamydomonas reinhardtii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.7.7.1 0.024
-
 oxidized ferredoxin
-
 Chlamydomonas reinhardtii
1.7.7.1 0.91
-
 methyl viologen
-
 Chlamydomonas reinhardtii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.7.7.1 Iron 0.65 molecules of siroheme per enzyme molecule Chlamydomonas reinhardtii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.7.7.1 25000
-
 1 * 63000 + 1 * 25000, SDS-PAGE Chlamydomonas reinhardtii
1.7.7.1 63000
-
 1 * 63000 + 1 * 25000, SDS-PAGE Chlamydomonas reinhardtii
1.7.7.1 86000
-
 gel filtration Chlamydomonas reinhardtii

Organism

EC Number Organism UniProt Comment Textmining
1.7.7.1 Chlamydomonas reinhardtii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.7.7.1
-
 Chlamydomonas reinhardtii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.7.7.1 89
-
-
 Chlamydomonas reinhardtii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.7.1 NH3 + H2O + oxidized ferredoxin
-
 Chlamydomonas reinhardtii nitrite + reduced ferredoxin + H+
-
 r
1.7.7.1 nitrite + reduced ferredoxin other electron donor: methyl viologen Chlamydomonas reinhardtii ammonia + oxidized ferredoxin
-
 ?
1.7.7.1 nitrite + reduced ferredoxin other electron donor: benzyl viologen Chlamydomonas reinhardtii ammonia + oxidized ferredoxin
-
 ?
1.7.7.1 nitrite + reduced methyl viologen
-
 Chlamydomonas reinhardtii NH3 + H2O + oxidized methyl viologen
-
 ?

Subunits

EC Number Subunits Comment Organism
1.7.7.1 dimer 1 * 63000 + 1 * 25000, SDS-PAGE Chlamydomonas reinhardtii

Cofactor

EC Number Cofactor Comment Organism Structure
1.7.7.1 siroheme 0.65 mol of siroheme per mol of enzyme Chlamydomonas reinhardtii