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Literature summary extracted from

  • Hayashi, M.; Hasegawa, K.; Oguni, Y.; Unemoto, T.
    Characterization of FMN-dependent NADH-quinone reductase induced by menadione in Escherichia coli (1990), Biochim. Biophys. Acta, 1035, 230-236.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
7.1.1.2 3,3'-methylene-bis(4-hydroxycoumarin)
-
 Escherichia coli
7.1.1.2 dicoumarol
-
 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
7.1.1.2 0.002
-
 menadione pH 7.5, 30°C Escherichia coli
7.1.1.2 0.132
-
 NADH pH 7.5, 30°C Escherichia coli

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
7.1.1.2 24000
-
 2 * 24000, SDS-PAGE Escherichia coli
7.1.1.2 50000
-
 gel filtration Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
7.1.1.2 additional information Escherichia coli FMN-dependent NADH-quinone reductase induced by menadione ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
7.1.1.2 Escherichia coli
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7.1.1.2 2 ferricyanide + NADH
-
 Escherichia coli 2 ferrocyanide + NAD+ + H+
-
 ?
7.1.1.2 2,3-dimethyl-1,4-naphthoquinone + NADH + H+
-
 Escherichia coli 2,3-dimethyl-1,4-hydronaphthoquinol + NAD+
-
 ?
7.1.1.2 2-methyl-1,4-naphthoquinone + NADH + H+ specific for NADH Escherichia coli 2-methyl-1,4-naphthoquinol + NAD+
-
 ?
7.1.1.2 2-methyl-1,4-naphthoquinone + NADH + H+ i.e. menadione Escherichia coli 2-methyl-1,4-naphthoquinol + NAD+
-
 ?
7.1.1.2 benzoquinone + NADH + H+
-
 Escherichia coli benzohydroquinone + NAD+
-
 ?
7.1.1.2 additional information no activity with coenzyme Q10 Escherichia coli ?
-
 ?
7.1.1.2 additional information FMN-dependent NADH-quinone reductase induced by menadione Escherichia coli ?
-
 ?
7.1.1.2 oxidized 2,6-dichlorophenolindophenol + NADH + H+
-
 Escherichia coli reduced 2,6-dichlorophenolindophenol + NAD+
-
 ?
7.1.1.2 ubiquinone-1 + NADH + H+
-
 Escherichia coli ubiquinol-1 + NAD+
-
 ?

Subunits

EC Number Subunits Comment Organism
7.1.1.2 dimer 2 * 24000, SDS-PAGE Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
7.1.1.2 NADH-quinone reductase
-
 Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
7.1.1.2 7 8
-
 Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
7.1.1.2 FAD requires FMN or FAD as cofactor, FMN is more effective. Half-maximum activity is obtained with 0.00054 mM FMN or 0.0165 mM FAD Escherichia coli
7.1.1.2 FMN enzyme requires FMN or FAD as cofactor, FMN is 30times more effective. Half-maximum activity is obtained with 0.00054 mM FMN or 0.0165 mM FAD Escherichia coli
7.1.1.2 NADH no activity with NADPH Escherichia coli

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
7.1.1.2 0.00022
-
 dicoumarol pH 7.5, 30°C Escherichia coli