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Literature summary extracted from

  • Petitdemange, H.; Marczak, R.; Raval, G.; Gay, R.
    Menadione reductase from Clostridium tyrobutyricum (1980), Can. J. Microbiol., 26, 324-329.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.6.5.2 3,3'-methylene-bis(4-hydroxycoumarin)
-
 Clostridium tyrobutyricum
1.6.5.2 additional information not inhibited by o-phenanthroline, KCN, quinacrine, EDTA and 2,3-dimercapto-1-propanol Clostridium tyrobutyricum
1.6.5.2 p-chloromercuribenzoate
-
 Clostridium tyrobutyricum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.6.5.2 0.0025
-
 FMN
-
 Clostridium tyrobutyricum
1.6.5.2 0.035
-
 menadione cofactor NADH Clostridium tyrobutyricum
1.6.5.2 0.223
-
 NADH cofactor menadione Clostridium tyrobutyricum
1.6.5.2 0.34
-
 NADPH acceptor menadione Clostridium tyrobutyricum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.6.5.2 60000
-
 gel filtration Clostridium tyrobutyricum

Organism

EC Number Organism UniProt Comment Textmining
1.6.5.2 Clostridium tyrobutyricum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.6.5.2
-
 Clostridium tyrobutyricum

Reaction

EC Number Reaction Comment Organism Reaction ID
1.6.5.2 NAD(P)H + H+ + a quinone = NAD(P)+ + a hydroquinone ping-pong mechanism Clostridium tyrobutyricum
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.6.5.2 46.2
-
-
 Clostridium tyrobutyricum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.6.5.2 1,4-naphthoquinone + NAD(P)H
-
 Clostridium tyrobutyricum 1,4-naphthohydroquinone + NAD(P)+
-
 ?
1.6.5.2 benzoquinone + 2 NAD(P)H + H+ no activity with coenzyme Q6 and vitamin K1 Clostridium tyrobutyricum benzohydroquinone + 2 NAD(P)+
-
 ?
1.6.5.2 menadione + NADH + H+
-
 Clostridium tyrobutyricum menadiol + NAD+
-
 r
1.6.5.2 NAD(P)H + H+ + oxidized 2,6-dichlorophenolindophenol low activity Clostridium tyrobutyricum NAD(P)+ + reduced 2,6-dichlorophenolindophenol
-
 ?

Subunits

EC Number Subunits Comment Organism
1.6.5.2 monomer 1 * 60000, SDS-PAGE Clostridium tyrobutyricum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.6.5.2 4.4
-
 plateau region from pH 6.0-7.5 with 70% activity Clostridium tyrobutyricum

Cofactor

EC Number Cofactor Comment Organism Structure
1.6.5.2 FMN contains FNN as prosthetic group Clostridium tyrobutyricum
1.6.5.2 NADH
-
 Clostridium tyrobutyricum
1.6.5.2 NADPH
-
 Clostridium tyrobutyricum