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Literature summary extracted from

  • Di Prisco, G.; Casola, L.; Giuditta, A.
    Purification and properties of a soluble reduced nicotinamide-adenine dinucleotide (phosphate) dehydrogenase from the hepatopancreas of Octopus vulgaris (1967), Biochem. J., 105, 455-460.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.6.5.2 2-mercaptoethanol stimulates Octopus vulgaris
1.6.5.2 cysteine stimulates Octopus vulgaris
1.6.5.2 GSH maximal stimulation with 0.5 mM, approx. 3fold activity Octopus vulgaris

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.6.5.2 3,3'-methylene-bis(4-hydroxycoumarin) only in the presence of glutathione Octopus vulgaris

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.6.5.2 0.034
-
 NADH
-
 Octopus vulgaris
1.6.5.2 0.22
-
 NADPH
-
 Octopus vulgaris

Organism

EC Number Organism UniProt Comment Textmining
1.6.5.2 Octopus vulgaris
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.6.5.2
-
 Octopus vulgaris

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.6.5.2 hepatopancreas
-
 Octopus vulgaris
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.6.5.2 2.8
-
-
 Octopus vulgaris

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.6.5.2 benzoquinone + 2 NAD(P)H + H+ no reaction with p-benzoquinone Octopus vulgaris benzohydroquinone + 2 NAD(P)+
-
 ?
1.6.5.2 ferricyanide + NADH + H+
-
 Octopus vulgaris ferrocyanide + NAD+
-
 r
1.6.5.2 K3[Fe(CN)6] + NAD(P)H no activity with O2, vitamin K3, ubiquinone-30 and 2-p-iodophenyl-3-p-nitrophenyl-5-phenyltetrazolium chloride Octopus vulgaris K4[Fe(CN)6] + NAD(P)+
-
 ?
1.6.5.2 K3[Fe(CN)6] + NAD(P)H no activity with lipoic acid, cytochrome c and vitamin K1 Octopus vulgaris K4[Fe(CN)6] + NAD(P)+
-
 ?
1.6.5.2 NAD(P)H + H+ + oxidized 2,6-dichlorophenolindophenol low activity Octopus vulgaris NAD(P)+ + reduced 2,6-dichlorophenolindophenol
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
1.6.5.2 FMN contains FNN as prosthetic group Octopus vulgaris

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.6.5.2 0.058
-
 dicoumarol cofactor NADH Octopus vulgaris
1.6.5.2 0.062
-
 dicoumarol cofactor NADPH Octopus vulgaris