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Literature summary extracted from

  • Wosilait, W.D.
    The reduction of vitamin K1 by an enzyme from dog liver (1960), J. Biol. Chem., 235, 1196-1201.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.6.5.2 stable to dialysis against 0.01 M potassium phosphate buffer, pH 7.5 and 0.01 M Tris buffer, pH 8.2 and 8.9, 16 h or more at 3°C Canis lupus familiaris

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.6.5.2 1,10-phenanthroline 1 mM, 50% inhibition Canis lupus familiaris
1.6.5.2 2,4-Dinitroaniline 0.1 mM, 33% inhibition Canis lupus familiaris
1.6.5.2 2,4-Dinitronaphthol 0.1 mM, 90% inhibition Canis lupus familiaris
1.6.5.2 2,4-dinitrophenol 0.1 mM, 39% inhibition Canis lupus familiaris
1.6.5.2 3,3'-methylene-bis(4-hydroxycoumarin) 0.1 mM, 97% inhibition Canis lupus familiaris
1.6.5.2 8-hydroxyquinoline 1 mM, 50% inhibition Canis lupus familiaris
1.6.5.2 alpha,alpha'-dipyridyl IC50: 1 mM Canis lupus familiaris
1.6.5.2 ethyl bis(2-hydroxy-4-oxo-4H-chromen-3-yl)acetate trivial tromexan, 0.1 mM, 79% inhibition Canis lupus familiaris
1.6.5.2 Phenindione 0.1 mM, 92% inhibition Canis lupus familiaris
1.6.5.2 salicylate 1 mM, 62% inhibition Canis lupus familiaris
1.6.5.2 warfarin 1 mM, 70% inhibition Canis lupus familiaris

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.6.5.2 mitochondrion
-
 Canis lupus familiaris 5739
-

Organism

EC Number Organism UniProt Comment Textmining
1.6.5.2 Canis lupus familiaris
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.6.5.2
-
 Canis lupus familiaris

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.6.5.2 liver
-
 Canis lupus familiaris
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.6.5.2 2.9
-
-
 Canis lupus familiaris

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.6.5.2 2-methyl-1,4-naphthoquinone + NAD(P)H artificial acceptor, trivial name menadione Canis lupus familiaris 2-methyl-1,4-naphthoquinol + NAD(P)+
-
 ?
1.6.5.2 2-methyl-3-phytyl-1,4-naphthoquinone + NAD(P)H trivial name vitamin K1 Canis lupus familiaris 2-methyl-3-phytyl-1,4-naphthohydroquinone + NAD(P)+
-
 ?
1.6.5.2 2-methyl-3-phytyl-1,4-naphthoquinone + NAD(P)H no activity with vitamin K1 diphosphate, menadione diphosphate, vitamin K1 oxide, d-alpha-tocopherol and d-alpha-tocoquinone Canis lupus familiaris 2-methyl-3-phytyl-1,4-naphthohydroquinone + NAD(P)+
-
 ?
1.6.5.2 benzoquinone + 2 NAD(P)H + H+
-
 Canis lupus familiaris benzohydroquinone + 2 NAD(P)+
-
 ?
1.6.5.2 coenzyme Q10 + NAD(P)H
-
 Canis lupus familiaris reduced coenzyme Q10 + NAD(P)+
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.6.5.2
-
-
 some days Canis lupus familiaris
1.6.5.2 20
-
 several h Canis lupus familiaris
1.6.5.2 57
-
 5 min, 90% loss of activity Canis lupus familiaris

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.6.5.2 9.7
-
 decrease above Canis lupus familiaris

Cofactor

EC Number Cofactor Comment Organism Structure
1.6.5.2 deamino-NADH
-
 Canis lupus familiaris
1.6.5.2 FAD stimulates Canis lupus familiaris
1.6.5.2 FMN stimulation Canis lupus familiaris
1.6.5.2 NADH
-
 Canis lupus familiaris
1.6.5.2 NADPH
-
 Canis lupus familiaris

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.6.5.2 1
-
 IC50: 1 mM Canis lupus familiaris alpha,alpha'-dipyridyl