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Literature summary extracted from

  • Capuozzo, E.; Crifo, C.; Salerno, C.
    Polyene antibiotics inhibit superoxide-producing NADPH oxidase in a polymorphonuclear cell-free system (1993), Biochem. Pharmacol., 45, 766-768.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.6.3.1 arachidonic acid maximal enzyme activity in the presence of 0.25-0.35 mM, inhibition above Bos taurus
1.6.3.1 GTP-gammaS optimal concentration approx. 0.015 mM Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.6.3.1 lucensomycin 0.02 mM, 50% inhibition Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.6.3.1 membrane
-
 Bos taurus 16020
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.6.3.1 NADPH + O2 Bos taurus
-
 NADP+ + O2-
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.6.3.1 Bos taurus
-
 multienzyme complex
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.6.3.1 neutrophil resting neutrophils Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.6.3.1 0.015
-
 activity in membranes after 1 h at 25°C Bos taurus
1.6.3.1 0.033
-
 activity in freshly prepared membranes at 25°C Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.6.3.1 NADPH + O2
-
 Bos taurus NADP+ + O2-
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.6.3.1 25
-
 activity in membranes, approx. 50% activity are lost after 1 h, freeze/thawing accelerates inactivation, loss of 90% activity after 1 h Bos taurus