EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.7.99.1 | Guanidinium chloride | 25% inhibition at 2 mM, 30% inhibition at 3 mM | Cucurbita pepo |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.7.99.1 | 35000 | - |
hydroxylamine reductase 2, gel filtration | Cucurbita pepo |
1.7.99.1 | 60000 | - |
hydroxylamine reductase 1, gel filtration | Cucurbita pepo |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.7.99.1 | Cucurbita pepo | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.7.99.1 | - |
Cucurbita pepo |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.7.99.1 | leaf | - |
Cucurbita pepo | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.7.99.1 | additional information | - |
specific activities after different purification methods | Cucurbita pepo |
EC Number | Storage Stability | Organism |
---|---|---|
1.7.99.1 | -18°C, DEAE-cellulose fraction, often stable for several weeks, but unpredictable loss of activity | Cucurbita pepo |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.7.99.1 | hydroxylamine + reduced acceptor | hydroxylamine reductase 1 reacts with reduced benzyl viologen, ferredoxin and FMNH2 | Cucurbita pepo | ammonia + oxidized acceptor | - |
? | |
1.7.99.1 | hydroxylamine + reduced acceptor | hydroxylamine reductase 2 reacts with benzyl viologen and dithionite | Cucurbita pepo | ammonia + oxidized acceptor | - |
? |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.7.99.1 | 55 | - |
10 min, 25-40% loss of activity | Cucurbita pepo |
1.7.99.1 | 60 | - |
10 min, 50-85% loss of activity | Cucurbita pepo |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.7.99.1 | additional information | - |
no clear or consistent pH optima observed with hydroxylamine reductase 1 and hydroxylamine reductase 2 | Cucurbita pepo |