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Literature summary extracted from

  • Takesue, S.; Omura, T.
    Purification and properties of NADH-cytochrome b5 reductase solubilized by lysosomes from rat liver microsomes (1970), J. Biochem., 67, 267-276.
    View publication on PubMed

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.6.2.2 endoplasmic reticulum
-
 Rattus norvegicus 5783
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.6.2.2 27000
-
 sedimentation equilibrium Rattus norvegicus
1.6.2.2 28000
-
 gel filtration Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
1.6.2.2 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.6.2.2 lysosome-solubilization Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.6.2.2 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.6.2.2 2480
-
 reduction of ferricyanide Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
1.6.2.2 frozen, 50 mM potassium phosphate buffer, pH 7.5, 1 mM EDTA, several weeks Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.6.2.2 2 ferricytochrome b5 + NADH specific for NADH as electron donor, artificial acceptors: ferricyanide, 2,6-dichlorphenolindophenol Rattus norvegicus 2 ferrocytochrome b5 + NAD+ + H+
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
1.6.2.2 NADH
-
 Rattus norvegicus
1.6.2.2 NADPH 1/300 of NADH linked activity Rattus norvegicus