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Literature summary extracted from

  • Yubisui, T.; Takeshita, M.
    Purification and properties of soluble NADH-cytochrome b5 reductase of rabbit erythrocytes (1982), J. Biochem., 91, 1467-1477.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.6.2.2 Atebrin 0.5 mM, complete inhibition Oryctolagus cuniculus
1.6.2.2 iodoacetamide 1 mM, complete inhibition Oryctolagus cuniculus
1.6.2.2 N-ethylmaleimide 1 mM, 90% inhibition Oryctolagus cuniculus
1.6.2.2 p-chloromercuribenzoate 0.001 mM, complete inhibition Oryctolagus cuniculus
1.6.2.2 Proflavin 0.1 mM, 74% inhibition Oryctolagus cuniculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.6.2.2 0.0025
-
 NADH
-
 Oryctolagus cuniculus
1.6.2.2 0.009
-
 ferricytochrome b5
-
 Oryctolagus cuniculus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.6.2.2 additional information rate of reduction depends on ionic strength Oryctolagus cuniculus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.6.2.2 33000
-
 gel filtration Oryctolagus cuniculus
1.6.2.2 34700
-
 erythrocytes, cytosol, gel filtration, calculation from FAD content Oryctolagus cuniculus

Organism

EC Number Organism UniProt Comment Textmining
1.6.2.2 Oryctolagus cuniculus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.6.2.2
-
 Oryctolagus cuniculus

Reaction

EC Number Reaction Comment Organism Reaction ID
1.6.2.2 NADH + 2 ferricytochrome b5 = NAD+ + H+ + 2 ferrocytochrome b5 ordered bi bi mechanism Oryctolagus cuniculus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.6.2.2 erythrocyte soluble form Oryctolagus cuniculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.6.2.2 497
-
-
 Oryctolagus cuniculus

Storage Stability

EC Number Storage Stability Organism
1.6.2.2 -20°C, 25 mM phosphate buffer, pH 7.6, 1 mM EDTA, 0.1 mM dithiothreitol, at least a few months Oryctolagus cuniculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.6.2.2 2 ferricytochrome b5 + NADH specific for NADH as electron donor, artificial acceptors: ferricyanide, 2,6-dichlorphenolindophenol Oryctolagus cuniculus 2 ferrocytochrome b5 + NAD+ + H+
-
 ?
1.6.2.2 2 ferricytochrome b5 + NADH additional acceptor: hemin Oryctolagus cuniculus 2 ferrocytochrome b5 + NAD+ + H+
-
 ?
1.6.2.2 2 ferricytochrome b5 + NADH poor donor: NADPH Oryctolagus cuniculus 2 ferrocytochrome b5 + NAD+ + H+
-
 ?
1.6.2.2 NADH + ferricytochrome b5
-
 Oryctolagus cuniculus NAD+ + H+ + ferrocytochrome b5
-
 r

Subunits

EC Number Subunits Comment Organism
1.6.2.2 monomer 1 * 33000, soluble enzyme from erythrocyte, SDS-PAGE Oryctolagus cuniculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.6.2.2 additional information
-
 no distinct optimum with phosphate or Tris-HCl buffer Oryctolagus cuniculus
1.6.2.2 6.4
-
 citrate-phosphate buffer Oryctolagus cuniculus
1.6.2.2 6.6
-
 Tris-maleate buffer Oryctolagus cuniculus

Cofactor

EC Number Cofactor Comment Organism Structure
1.6.2.2 FAD 1 mol FAD per mol enzyme Oryctolagus cuniculus
1.6.2.2 NADH
-
 Oryctolagus cuniculus