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Literature summary extracted from

  • Machida, Y.; Nakanishi, T.
    Purification and properties of uricase from Enterobacter cloacae (1980), Agric. Biol. Chem., 44, 2811-2815.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.7.3.3 Ag+
-
 Enterobacter cloacae
1.7.3.3 CN-
-
 Enterobacter cloacae
1.7.3.3 Co2+
-
 Enterobacter cloacae
1.7.3.3 Cu2+
-
 Enterobacter cloacae
1.7.3.3 N-ethylmaleimide
-
 Enterobacter cloacae
1.7.3.3 p-chloromercuribenzoate
-
 Enterobacter cloacae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.7.3.3 1.5
-
 uric acid
-
 Enterobacter cloacae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.7.3.3 cell associated
-
 Enterobacter cloacae
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.7.3.3 105000
-
 gel filtration Enterobacter cloacae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.7.3.3 urate + O2 + H2O Enterobacter cloacae
-
 5-hydroxyisourate + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.7.3.3 Enterobacter cloacae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.7.3.3
-
 Enterobacter cloacae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.7.3.3 6.6
-
-
 Enterobacter cloacae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.3.3 urate + O2 + H2O
-
 Enterobacter cloacae 5-hydroxyisourate + H2O2
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.7.3.3 40
-
-
 Enterobacter cloacae

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.7.3.3 20 50 20°C: about 60% of activity maximum Enterobacter cloacae
1.7.3.3 20 50 50°C: about 50% of activity maximum Enterobacter cloacae

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.7.3.3 40
-
 30 min, stable below Enterobacter cloacae
1.7.3.3 60
-
 30 min, about 40% loss of activity Enterobacter cloacae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.7.3.3 9.5
-
-
 Enterobacter cloacae

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.7.3.3 7 11 pH 7: about 50% of activity maximum, pH 11: about 40% of activity maximum Enterobacter cloacae

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.7.3.3 6
-
 45°C, 30 min, 40% loss of activity Enterobacter cloacae
1.7.3.3 8 9 45°C, 30 min, stable Enterobacter cloacae
1.7.3.3 10
-
 45°C, 30 min, 30% loss of activity Enterobacter cloacae