EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.8.2.2 | cyanide | not inhibitory | Thiobacillus sp. | |
1.8.2.2 | EDTA | not inhibitory | Thiobacillus sp. | |
1.8.2.2 | N-ethylmaleimide | not inhibitory | Thiobacillus sp. | |
1.8.2.2 | Na2SeO3 | it inhibits the oxidation of thiosulfate by whole cells | Thiobacillus sp. | |
1.8.2.2 | SO32- | inhibits at concentrations above 0.05 mM, the sulfite inhibition of thiosulfate oxidation by whole cells is very similar to the inhibition pattern of the enzyme | Thiobacillus sp. |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.8.2.2 | 0.12 | - |
thiosulfate | at pH 7.5 | Thiobacillus sp. |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.8.2.2 | periplasm | at least 73% of the enzyme activity is recovered in the periplasmic fraction | Thiobacillus sp. | - |
- |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.8.2.2 | 27000 | - |
2 * 33000 + 2 * 27000, SDS-PAGE | Thiobacillus sp. |
1.8.2.2 | 33000 | - |
2 * 33000 + 2 * 27000, SDS-PAGE | Thiobacillus sp. |
1.8.2.2 | 120000 | - |
gel filtration | Thiobacillus sp. |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.8.2.2 | Thiobacillus sp. | - |
W5 | - |
1.8.2.2 | Thiobacillus sp. W5 | - |
W5 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.8.2.2 | using chromatography on Phenyl-Sepharose column and Mono Q column, gel filtration on Superdex 200 and column chromatography on Phenyl Superose | Thiobacillus sp. |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.8.2.2 | 1149 | - |
purified enzyme | Thiobacillus sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.8.2.2 | additional information | no activity with: benzyl viologen, 2,6-dichloroindophenol, 2,6-dichloroindophenol with phenazine methosulfate, 2,6-dichloroindophenol with phenazine ethosulfate, horse heart cytochrome c with phenazine methosulfate, horse heart cytochrome c with phenazine ethosulfate | Thiobacillus sp. | ? | - |
? | |
1.8.2.2 | additional information | NAD+, methylene blue, 2,6-dichlorophenol indophenol | Thiobacillus sp. | ? | - |
? | |
1.8.2.2 | additional information | no activity with: benzyl viologen, 2,6-dichloroindophenol, 2,6-dichloroindophenol with phenazine methosulfate, 2,6-dichloroindophenol with phenazine ethosulfate, horse heart cytochrome c with phenazine methosulfate, horse heart cytochrome c with phenazine ethosulfate | Thiobacillus sp. W5 | ? | - |
? | |
1.8.2.2 | additional information | NAD+, methylene blue, 2,6-dichlorophenol indophenol | Thiobacillus sp. W5 | ? | - |
? | |
1.8.2.2 | thiosulfate + oxidized acceptor | strictly specific for thiosulfate | Thiobacillus sp. | tetrathionate + reduced acceptor | - |
? | |
1.8.2.2 | thiosulfate + oxidized acceptor | the activity does not couple with mammalian cytochrome c as electron acceptor | Thiobacillus sp. | tetrathionate + reduced acceptor | - |
? | |
1.8.2.2 | thiosulfate + oxidized acceptor | ferricyanide acts as electron acceptor | Thiobacillus sp. | tetrathionate + reduced acceptor | - |
? | |
1.8.2.2 | thiosulfate + oxidized acceptor | strictly specific for thiosulfate | Thiobacillus sp. W5 | tetrathionate + reduced acceptor | - |
? | |
1.8.2.2 | thiosulfate + oxidized acceptor | the activity does not couple with mammalian cytochrome c as electron acceptor | Thiobacillus sp. W5 | tetrathionate + reduced acceptor | - |
? | |
1.8.2.2 | thiosulfate + oxidized acceptor | ferricyanide acts as electron acceptor | Thiobacillus sp. W5 | tetrathionate + reduced acceptor | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.8.2.2 | heterotetramer | 2 * 33000 + 2 * 27000, SDS-PAGE | Thiobacillus sp. |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.8.2.2 | 2300 | - |
thiosulfate | - |
Thiobacillus sp. |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.8.2.2 | 5.5 | - |
- |
Thiobacillus sp. |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.8.2.2 | heme c | 4 mol per mol of enzyme, present in both subunit types | Thiobacillus sp. |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.8.2.2 | 0.03 | - |
Na2SeO3 | it is possible that selenite blocks access to the thiosulfate dehydrogenase or has an inhibiting effect on the respiratory chain itself | Thiobacillus sp. | |
1.8.2.2 | 0.3 | - |
SO32- | sulfite resembles the sulfonate part of thiosulfate and might, therefore, block the active site of the enzyme | Thiobacillus sp. |