Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Silver, M.; Lundgren, D.G.
    The thiosulfate-oxidizing enzyme of Ferrobacillus ferrooxidans (Thiobacillus ferrooxidans) (1968), Can. J. Biochem., 46, 1215-1220.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.8.2.2 azide 60% inhibition at 10 mM Acidithiobacillus ferrooxidans
1.8.2.2 cyanide 28.9% inhibition at 10 mM Acidithiobacillus ferrooxidans
1.8.2.2 HgCl2 73.3% inhibition at 1 mM Acidithiobacillus ferrooxidans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.2.2 additional information
-
 additional information
-
 Acidithiobacillus ferrooxidans
1.8.2.2 0.9
-
 thiosulfate
-
 Acidithiobacillus ferrooxidans

Organism

EC Number Organism UniProt Comment Textmining
1.8.2.2 Acidithiobacillus ferrooxidans
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.2.2 using heat-treatment, 5-17% ethanol precipitation and CM-cellulose treatment Acidithiobacillus ferrooxidans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.8.2.2 additional information
-
-
 Acidithiobacillus ferrooxidans

Storage Stability

EC Number Storage Stability Organism
1.8.2.2 -20°C, little loss of activity after 2 months Acidithiobacillus ferrooxidans
1.8.2.2 diluted enzyme loses 10-15% of the activity each time it is frozen and thawed Acidithiobacillus ferrooxidans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.2.2 thiosulfate + oxidized acceptor the activity does not couple with mammalian cytochrome c as electron acceptor Acidithiobacillus ferrooxidans tetrathionate + reduced acceptor
-
 ir
1.8.2.2 thiosulfate + oxidized acceptor ferricyanide acts as electron acceptor Acidithiobacillus ferrooxidans tetrathionate + reduced acceptor
-
 ir
1.8.2.2 thiosulfate + oxidized acceptor the enzyme is active only with ferricyanide, not with native or mammalian cytochrome c Acidithiobacillus ferrooxidans tetrathionate + reduced acceptor
-
 ir

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.8.2.2 100
-
 complete loss of activity Acidithiobacillus ferrooxidans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.8.2.2 5
-
-
 Acidithiobacillus ferrooxidans

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.8.2.2 4
-
 unstable below Acidithiobacillus ferrooxidans