Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Hansel, A.; Kuschel, L.; Hehl, S.; Lemke, C.; Agricola, H.J.; Hoshi, T.; Heinemann, S.H.
    Mitochondrial targeting of the human peptide methionine sulfoxide reductase (MSRA), an enzyme involved in the repair of oxidized proteins (2002), FASEB J., 16, 911-913.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.8.4.11 expression of GFP-tagged wild-type MsrA and several GFP-tagged deletion mutants in several mammalian cell lines, overview, expression of His-tagged wild-type and DELTA(1-22) deletion mutant in Escherichia coli strain M15 Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.8.4.11 mitochondrion MsrA contains a mitochondrial targeting sequence which is not necessary for catalytic activity Homo sapiens 5739
-
1.8.4.11 additional information subcellular localization analysis, the subcellular localization is not tissue-dependent Homo sapiens
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.8.4.11 peptide-L-methionine-(S)-S-oxide + thioredoxin Homo sapiens stereospecific reduction, the enzyme is involved in repair of oxidized proteins peptide-L-methionine + thioredoxin disulfide + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.8.4.11 Homo sapiens Q9UJ68
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.4.11 recombinant His-tagged wild-type and DELTA(1-22) deletion mutant from Escherichia coli strain M15 by nickel affinity chromatography Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.4.11 peptide-L-methionine-(S)-S-oxide + DTT stereospecific reduction Homo sapiens peptide-L-methionine + DTT disulfide + H2O
-
 ?
1.8.4.11 peptide-L-methionine-(S)-S-oxide + thioredoxin stereospecific reduction Homo sapiens peptide-L-methionine + thioredoxin disulfide + H2O
-
 ?
1.8.4.11 peptide-L-methionine-(S)-S-oxide + thioredoxin stereospecific reduction, the enzyme is involved in repair of oxidized proteins Homo sapiens peptide-L-methionine + thioredoxin disulfide + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.8.4.11 MsrA
-
 Homo sapiens
1.8.4.11 peptide methionine sulfoxide reductase
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.8.4.11 37
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.8.4.11 7.4
-
 assay at Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.4.11 dithiothreitol
-
 Homo sapiens
1.8.4.11 thioredoxin
-
 Homo sapiens