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Literature summary extracted from

  • Olry, A.; Boschi-Muller, S.; Marraud, M.; Sanglier-Cianferani, S.; Van Dorsselear, A.; Branlant, G.
    Characterization of the methionine sulfoxide reductase activities of PILB, a probable virulence factor from Neisseria meningitidis (2002), J. Biol. Chem., 277, 12016-12022.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.8.4.11 overexpression of wild-type and mutant enzymes in Escherichia coli Neisseria meningitidis
1.8.4.12 overexpression of wild-type and mutant enzymes in Escherichia coli Neisseria meningitidis

Protein Variants

EC Number Protein Variants Comment Organism
1.8.4.11 C206S site-directed mutagenesis, MsrA domain of PILB, inactive mutant Neisseria meningitidis
1.8.4.11 C348S site-directed mutagenesis, MsrA domain of PILB, mutant is inactive with thioredoxin, but about 10fold more active than the wild-type enzyme MsrA domain Neisseria meningitidis
1.8.4.12 C439S site-directed mutagenesis, MsrA domain of PILB, mutant is inactive with thioredoxin, but about 10fold more active than the wild-type enzyme MsrA domain Neisseria meningitidis
1.8.4.12 C494S site-directed mutagenesis, MsrA domain of PILB, inactive mutant Neisseria meningitidis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.4.11 additional information
-
 additional information kinetics Neisseria meningitidis
1.8.4.11 0.075
-
 thioredoxin MsrA activity of PILB, pH 8.0, 25°C Neisseria meningitidis
1.8.4.11 9
-
 L-methionine (R,S)-sulfoxide MsrB activity of PILB, pH 8.0, 25°C Neisseria meningitidis
1.8.4.12 additional information
-
 additional information kinetics Neisseria meningitidis
1.8.4.12 0.034
-
 thioredoxin MsrB activity of PILB, pH 8.0, 25°C Neisseria meningitidis
1.8.4.12 56
-
 L-methionine (R,S)-sulfoxide MsrB activity of PILB, pH 8.0, 25°C Neisseria meningitidis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.8.4.11 additional information content of free cysteinyl residues in wild-type and mutant enzymes, MsrA and MsrB domains, overview Neisseria meningitidis
1.8.4.12 additional information content of free cysteinyl residues in wild-type and mutant enzymes, MsrA and MsrB domains, overview Neisseria meningitidis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.8.4.11 21898
-
 x * 21898, about, recombinant MsrA domain, mass spectrometry Neisseria meningitidis
1.8.4.12 16374
-
 x * 16374, about, recombinant wild-type MsrB domain, mass spectrometry Neisseria meningitidis

Organism

EC Number Organism UniProt Comment Textmining
1.8.4.11 Neisseria meningitidis
-
 PilB enzyme has 2 catalytic domains showing MsrA, methionine S-oxide reductase (S-form oxidizing), and MsrB, methionine S-oxide reductase (R-form oxidizing), activity, respectively
-
1.8.4.12 Neisseria meningitidis
-
 PilB enzyme has 2 catalytic domains showing MsrA, methionine S-oxide reductase (S-form oxidizing), and MsrB, methionine S-oxide reductase (R-form oxidizing), activity, respectively
-

Reaction

EC Number Reaction Comment Organism Reaction ID
1.8.4.11 L-methionine (S)-sulfoxide + thioredoxin = L-methionine + thioredoxin disulfide + H2O reaction mechanism Neisseria meningitidis
a
1.8.4.12 L-methionine (R)-sulfoxide + thioredoxin = L-methionine + thioredoxin disulfide + H2O reaction mechanism, Cys494 and Cys439 are involved Neisseria meningitidis
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.8.4.11 3
-
 recombinant wild-type MsrA domain, cosubstrate dithiothreitol Neisseria meningitidis
1.8.4.11 4.2
-
 recombinant wild-type MsrA/MsrB, cosubstrate dithiothreitol Neisseria meningitidis
1.8.4.11 170
-
 recombinant wild-type MsrA/MsrB, cosubstrate thioredoxin Neisseria meningitidis
1.8.4.11 220
-
 recombinant wild-type MsrA domain, cosubstrate thioredoxin Neisseria meningitidis
1.8.4.12 3
-
 recombinant wild-type MsrB domain, cosubstrate dithiothreitol Neisseria meningitidis
1.8.4.12 4.2
-
 recombinant wild-type MsrA/MsrB, cosubstrate dithiothreitol Neisseria meningitidis
1.8.4.12 12
-
 recombinant wild-type MsrB domain, cosubstrate thioredoxin Neisseria meningitidis
1.8.4.12 170
-
 recombinant wild-type MsrA/MsrB, cosubstrate thioredoxin Neisseria meningitidis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.4.11 L-methionine (R,S)-sulfoxide + thioredoxin
-
 Neisseria meningitidis L-methionine + thioredoxin disulfide
-
 ?
1.8.4.11 L-methionine (S)-sulfoxide + thioredoxin MsrA activity of the tandem domains of PilB, the MsrA domain alone does not utilize the R-isomer Neisseria meningitidis L-methionine + thioredoxin disulfide + H2O
-
 ?
1.8.4.11 additional information substrate specificity of MsrA activity, diverse substrates, overview Neisseria meningitidis ?
-
 ?
1.8.4.12 L-methionine (R)-sulfoxide + thioredoxin MsrB activity of the tandem domains of PilB, the MsrB domain alone does not utilize the S-isomer Neisseria meningitidis L-methionine + thioredoxin disulfide + H2O
-
 ?
1.8.4.12 additional information substrate specificity of MsrB activity, diverse substrates, overview Neisseria meningitidis ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.8.4.11 ? x * 21898, about, recombinant MsrA domain, mass spectrometry Neisseria meningitidis
1.8.4.11 More MsrA activity is located on the central domain of PILB, the fused domains are folded entities Neisseria meningitidis
1.8.4.12 ? x * 16374, about, recombinant wild-type MsrB domain, mass spectrometry Neisseria meningitidis
1.8.4.12 More MsrB activity is located on the C-terminal domain of PILB, the fused domains are folded entities Neisseria meningitidis

Synonyms

EC Number Synonyms Comment Organism
1.8.4.11 methionine sulfoxide reductase
-
 Neisseria meningitidis
1.8.4.11 MsrA
-
 Neisseria meningitidis
1.8.4.11 PilB
-
 Neisseria meningitidis
1.8.4.12 methionine sulfoxide reductase
-
 Neisseria meningitidis
1.8.4.12 MsrB
-
 Neisseria meningitidis
1.8.4.12 PilB
-
 Neisseria meningitidis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.8.4.11 3.7
-
 L-methionine (S,R)-sulfoxide MsrB activity of PILB, pH 8.0, 25°C Neisseria meningitidis
1.8.4.12 2.8
-
 L-methionine (R,S)-sulfoxide MsrB activity of PILB, pH 8.0, 25°C Neisseria meningitidis

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.4.11 dithiothreitol
-
 Neisseria meningitidis
1.8.4.11 thioredoxin
-
 Neisseria meningitidis
1.8.4.12 dithiothreitol
-
 Neisseria meningitidis
1.8.4.12 thioredoxin
-
 Neisseria meningitidis