BRENDA - Enzyme Database show

Purification and properties of membrane-bound sulfite dehydrogenase from Thiobacillus thiooxidans JCM7814

Nakamura, K.; Yoshikawa, H.; Okubo, S.; Kurosawa, H.; Amano, Y.; Biosci. Biotechnol. Biochem. 59, 11-15 (1995)
No PubMed abstract available

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
1.8.2.1
Triton X-100
increases activity
Acidithiobacillus thiooxidans
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.8.2.1
arsenite
1 mM, complete inhibition
Acidithiobacillus thiooxidans
1.8.2.1
HgCl2
1 mM, 91% inhibition
Acidithiobacillus thiooxidans
1.8.2.1
monoiodoacetate
1 mM, 19% inhibition
Acidithiobacillus thiooxidans
1.8.2.1
NEM
1 mM, 53% inhibition
Acidithiobacillus thiooxidans
1.8.2.1
PCMB
1 mM, 47% loss of activity
Acidithiobacillus thiooxidans
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.8.2.1
1.95
-
sulfite
-
Acidithiobacillus thiooxidans
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.8.2.1
membrane
bound to
Acidithiobacillus thiooxidans
16020
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.8.2.1
62000
-
x * 62000 + x * 70000 + x * 74000, SDS-PAGE
Acidithiobacillus thiooxidans
1.8.2.1
70000
-
x * 62000 + x * 70000 + x * 74000, SDS-PAGE
Acidithiobacillus thiooxidans
1.8.2.1
74000
-
x * 62000 + x * 70000 + x * 74000, SDS-PAGE
Acidithiobacillus thiooxidans
1.8.2.1
400000
-
gel filtration
Acidithiobacillus thiooxidans
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.8.2.1
Acidithiobacillus thiooxidans
-
JCM7814
-
1.8.2.1
Acidithiobacillus thiooxidans JCM 7814
-
JCM7814
-
Purification (Commentary)
EC Number
Commentary
Organism
1.8.2.1
-
Acidithiobacillus thiooxidans
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.8.2.1
21
-
-
Acidithiobacillus thiooxidans
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.8.2.1
sulfite + ferricyanide + H2O
-
394088
Acidithiobacillus thiooxidans
sulfate + ferrocyanide + H+
-
-
-
?
1.8.2.1
sulfite + ferricyanide + H2O
-
394088
Acidithiobacillus thiooxidans JCM 7814
sulfate + ferrocyanide + H+
-
-
-
?
1.8.2.1
sulfite + ferricytochrome c + H2O
-
394088
Acidithiobacillus thiooxidans
sulfate + ferrocytochrome c + H+
-
-
-
?
1.8.2.1
sulfite + ferricytochrome c + H2O
-
394088
Acidithiobacillus thiooxidans JCM 7814
sulfate + ferrocytochrome c + H+
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.8.2.1
?
x * 62000 + x * 70000 + x * 74000, SDS-PAGE
Acidithiobacillus thiooxidans
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.8.2.1
20
-
-
Acidithiobacillus thiooxidans
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.8.2.1
30
-
1 h, stable below
Acidithiobacillus thiooxidans
1.8.2.1
40
-
1 h, about 20% loss of activity
Acidithiobacillus thiooxidans
1.8.2.1
50
-
1 h, complete loss of activity
Acidithiobacillus thiooxidans
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.8.2.1
7.5
-
-
Acidithiobacillus thiooxidans
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
1.8.2.1
7
-
20°C, 1 h, stable
Acidithiobacillus thiooxidans
1.8.2.1
9
-
20°C, 1 h, about 20% loss of activity
Acidithiobacillus thiooxidans
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
1.8.2.1
Triton X-100
increases activity
Acidithiobacillus thiooxidans
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.8.2.1
arsenite
1 mM, complete inhibition
Acidithiobacillus thiooxidans
1.8.2.1
HgCl2
1 mM, 91% inhibition
Acidithiobacillus thiooxidans
1.8.2.1
monoiodoacetate
1 mM, 19% inhibition
Acidithiobacillus thiooxidans
1.8.2.1
NEM
1 mM, 53% inhibition
Acidithiobacillus thiooxidans
1.8.2.1
PCMB
1 mM, 47% loss of activity
Acidithiobacillus thiooxidans
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.8.2.1
1.95
-
sulfite
-
Acidithiobacillus thiooxidans
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.8.2.1
membrane
bound to
Acidithiobacillus thiooxidans
16020
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.8.2.1
62000
-
x * 62000 + x * 70000 + x * 74000, SDS-PAGE
Acidithiobacillus thiooxidans
1.8.2.1
70000
-
x * 62000 + x * 70000 + x * 74000, SDS-PAGE
Acidithiobacillus thiooxidans
1.8.2.1
74000
-
x * 62000 + x * 70000 + x * 74000, SDS-PAGE
Acidithiobacillus thiooxidans
1.8.2.1
400000
-
gel filtration
Acidithiobacillus thiooxidans
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.8.2.1
-
Acidithiobacillus thiooxidans
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.8.2.1
21
-
-
Acidithiobacillus thiooxidans
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.8.2.1
sulfite + ferricyanide + H2O
-
394088
Acidithiobacillus thiooxidans
sulfate + ferrocyanide + H+
-
-
-
?
1.8.2.1
sulfite + ferricyanide + H2O
-
394088
Acidithiobacillus thiooxidans JCM 7814
sulfate + ferrocyanide + H+
-
-
-
?
1.8.2.1
sulfite + ferricytochrome c + H2O
-
394088
Acidithiobacillus thiooxidans
sulfate + ferrocytochrome c + H+
-
-
-
?
1.8.2.1
sulfite + ferricytochrome c + H2O
-
394088
Acidithiobacillus thiooxidans JCM 7814
sulfate + ferrocytochrome c + H+
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.8.2.1
?
x * 62000 + x * 70000 + x * 74000, SDS-PAGE
Acidithiobacillus thiooxidans
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.8.2.1
20
-
-
Acidithiobacillus thiooxidans
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.8.2.1
30
-
1 h, stable below
Acidithiobacillus thiooxidans
1.8.2.1
40
-
1 h, about 20% loss of activity
Acidithiobacillus thiooxidans
1.8.2.1
50
-
1 h, complete loss of activity
Acidithiobacillus thiooxidans
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.8.2.1
7.5
-
-
Acidithiobacillus thiooxidans
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
1.8.2.1
7
-
20°C, 1 h, stable
Acidithiobacillus thiooxidans
1.8.2.1
9
-
20°C, 1 h, about 20% loss of activity
Acidithiobacillus thiooxidans