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Enzymes involved in the metabolism of thiosulfate by Thiobacillus thioparus. I. Survey of enzymes and properties of sulfite: cytochrome c oxidoreductase

Lyric, R.M.; Suzuki, I.; Can. J. Biochem. 48, 334-343 (1970)

Data extracted from this reference:

General Stability
EC Number
General Stability
Organism
1.8.2.1
thawing and refreezing has no effect
Thiobacillus thioparus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.8.2.1
1,10-phenanthroline
5 mM, complete inhibition
Thiobacillus thioparus
1.8.2.1
2,2'-bipyridyl
5 mM, complete inhibition
Thiobacillus thioparus
1.8.2.1
AMP
-
Thiobacillus thioparus
1.8.2.1
arsenite
1 mM, 83% inhibition
Thiobacillus thioparus
1.8.2.1
Cl-
non-competitive
Thiobacillus thioparus
1.8.2.1
EDTA
4 mM complete inhibition
Thiobacillus thioparus
1.8.2.1
HgCl2
10 mM, complete inhibition
Thiobacillus thioparus
1.8.2.1
KCl
-
Thiobacillus thioparus
1.8.2.1
NaCl
noncompetitive
Thiobacillus thioparus
1.8.2.1
NEM
1 mM, 32% inhibition
Thiobacillus thioparus
1.8.2.1
PCMB
1 mM, complete inhibition
Thiobacillus thioparus
1.8.2.1
phosphate
50 mM, 90% inhibition, uncompetitive with respect to sulfite
Thiobacillus thioparus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.8.2.1
0.013
-
cytochrome c
-
Thiobacillus thioparus
1.8.2.1
0.088
-
sulfite
-
Thiobacillus thioparus
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.8.2.1
Iron
enzyme contains nonheme iron
Thiobacillus thioparus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.8.2.1
54000
-
sucrose density gradient method
Thiobacillus thioparus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.8.2.1
Thiobacillus thioparus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.8.2.1
-
Thiobacillus thioparus
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
1.8.2.1
0.69
-
-
Thiobacillus thioparus
Storage Stability
EC Number
Storage Stability
Organism
1.8.2.1
-20C, stable for several months
Thiobacillus thioparus
1.8.2.1
4C, 10-20% loss of activity after 6 h at room temperature, 80% loss of activity after 5 days
Thiobacillus thioparus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.8.2.1
sulfite + ferricyanide + H2O
-
394081
Thiobacillus thioparus
sulfate + ferrocyanide + H+
-
-
-
?
1.8.2.1
sulfite + ferricytochrome c + H2O
horse heart cytochrome or yeast cytochrome
394081
Thiobacillus thioparus
sulfate + ferrocytochrome c + H+
-
-
-
?
Temperature Stability [C]
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
1.8.2.1
55
-
9 min, 95% loss of activity
Thiobacillus thioparus
1.8.2.1
60
-
6 min, 95% loss of activity
Thiobacillus thioparus
1.8.2.1
100
-
1 min, complete loss of activity
Thiobacillus thioparus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.8.2.1
8
-
with Tricine-HCl or Tricine-NaOH buffer
Thiobacillus thioparus
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1.8.2.1
38
-
Cl-
-
Thiobacillus thioparus
General Stability (protein specific)
EC Number
General Stability
Organism
1.8.2.1
thawing and refreezing has no effect
Thiobacillus thioparus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.8.2.1
1,10-phenanthroline
5 mM, complete inhibition
Thiobacillus thioparus
1.8.2.1
2,2'-bipyridyl
5 mM, complete inhibition
Thiobacillus thioparus
1.8.2.1
AMP
-
Thiobacillus thioparus
1.8.2.1
arsenite
1 mM, 83% inhibition
Thiobacillus thioparus
1.8.2.1
Cl-
non-competitive
Thiobacillus thioparus
1.8.2.1
EDTA
4 mM complete inhibition
Thiobacillus thioparus
1.8.2.1
HgCl2
10 mM, complete inhibition
Thiobacillus thioparus
1.8.2.1
KCl
-
Thiobacillus thioparus
1.8.2.1
NaCl
noncompetitive
Thiobacillus thioparus
1.8.2.1
NEM
1 mM, 32% inhibition
Thiobacillus thioparus
1.8.2.1
PCMB
1 mM, complete inhibition
Thiobacillus thioparus
1.8.2.1
phosphate
50 mM, 90% inhibition, uncompetitive with respect to sulfite
Thiobacillus thioparus
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1.8.2.1
38
-
Cl-
-
Thiobacillus thioparus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.8.2.1
0.013
-
cytochrome c
-
Thiobacillus thioparus
1.8.2.1
0.088
-
sulfite
-
Thiobacillus thioparus
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.8.2.1
Iron
enzyme contains nonheme iron
Thiobacillus thioparus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.8.2.1
54000
-
sucrose density gradient method
Thiobacillus thioparus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.8.2.1
-
Thiobacillus thioparus
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
1.8.2.1
0.69
-
-
Thiobacillus thioparus
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
1.8.2.1
-20C, stable for several months
Thiobacillus thioparus
1.8.2.1
4C, 10-20% loss of activity after 6 h at room temperature, 80% loss of activity after 5 days
Thiobacillus thioparus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.8.2.1
sulfite + ferricyanide + H2O
-
394081
Thiobacillus thioparus
sulfate + ferrocyanide + H+
-
-
-
?
1.8.2.1
sulfite + ferricytochrome c + H2O
horse heart cytochrome or yeast cytochrome
394081
Thiobacillus thioparus
sulfate + ferrocytochrome c + H+
-
-
-
?
Temperature Stability [C] (protein specific)
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
1.8.2.1
55
-
9 min, 95% loss of activity
Thiobacillus thioparus
1.8.2.1
60
-
6 min, 95% loss of activity
Thiobacillus thioparus
1.8.2.1
100
-
1 min, complete loss of activity
Thiobacillus thioparus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.8.2.1
8
-
with Tricine-HCl or Tricine-NaOH buffer
Thiobacillus thioparus