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Literature summary extracted from

  • Lyric, R.M.; Suzuki, I.
    Enzymes involved in the metabolism of thiosulfate by Thiobacillus thioparus. I. Survey of enzymes and properties of sulfite: cytochrome c oxidoreductase (1970), Can. J. Biochem., 48, 334-343.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.8.2.1 thawing and refreezing has no effect Thiobacillus thioparus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.8.2.1 1,10-phenanthroline 5 mM, complete inhibition Thiobacillus thioparus
1.8.2.1 2,2'-bipyridyl 5 mM, complete inhibition Thiobacillus thioparus
1.8.2.1 AMP
-
 Thiobacillus thioparus
1.8.2.1 arsenite 1 mM, 83% inhibition Thiobacillus thioparus
1.8.2.1 Cl- non-competitive Thiobacillus thioparus
1.8.2.1 EDTA 4 mM complete inhibition Thiobacillus thioparus
1.8.2.1 HgCl2 10 mM, complete inhibition Thiobacillus thioparus
1.8.2.1 KCl
-
 Thiobacillus thioparus
1.8.2.1 NaCl noncompetitive Thiobacillus thioparus
1.8.2.1 NEM 1 mM, 32% inhibition Thiobacillus thioparus
1.8.2.1 PCMB 1 mM, complete inhibition Thiobacillus thioparus
1.8.2.1 phosphate 50 mM, 90% inhibition, uncompetitive with respect to sulfite Thiobacillus thioparus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.2.1 0.013
-
 ferricytochrome c
-
 Thiobacillus thioparus
1.8.2.1 0.088
-
 sulfite
-
 Thiobacillus thioparus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.8.2.1 Iron enzyme contains nonheme iron Thiobacillus thioparus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.8.2.1 54000
-
 sucrose density gradient method Thiobacillus thioparus

Organism

EC Number Organism UniProt Comment Textmining
1.8.2.1 Thiobacillus thioparus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.2.1
-
 Thiobacillus thioparus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.8.2.1 0.69
-
-
 Thiobacillus thioparus

Storage Stability

EC Number Storage Stability Organism
1.8.2.1 -20°C, stable for several months Thiobacillus thioparus
1.8.2.1 4°C, 10-20% loss of activity after 6 h at room temperature, 80% loss of activity after 5 days Thiobacillus thioparus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.2.1 sulfite + ferricyanide + H2O
-
 Thiobacillus thioparus sulfate + ferrocyanide + H+
-
 ?
1.8.2.1 sulfite + ferricytochrome c + H2O horse heart cytochrome or yeast cytochrome Thiobacillus thioparus sulfate + ferrocytochrome c + H+
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.8.2.1 55
-
 9 min, 95% loss of activity Thiobacillus thioparus
1.8.2.1 60
-
 6 min, 95% loss of activity Thiobacillus thioparus
1.8.2.1 100
-
 1 min, complete loss of activity Thiobacillus thioparus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.8.2.1 8
-
 with Tricine-HCl or Tricine-NaOH buffer Thiobacillus thioparus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.8.2.1 38
-
 Cl-
-
 Thiobacillus thioparus