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Literature summary extracted from

  • Moran, J.F.; Sun, Z.; Sarath, G.; Arredondo-Peter, R.; James, E.K.; Becana, M.; Klucas, R.V.
    Molecular cloning, functional characterization, and subcellular localization of soybean nodule dihydrolipoamide reductase (2002), Plant Physiol., 128, 300-313.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.6.2.6 overexpression in Escherichia coli BL21 Glycine max
1.8.1.4 overexpression in Escherichia coli Glycine max

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.6.2.6 0.029
-
 ferrileghemoglobin recombinant FLbR-2 Glycine max
1.6.2.6 0.058
-
 NADH recombinant FLbR-2 Glycine max
1.6.2.6 3.381
-
 Lipoamide recombinant FLbR-2 Glycine max
1.8.1.4 0.029
-
 ferrileghemoglobin
-
 Glycine max
1.8.1.4 0.058
-
 NADH
-
 Glycine max
1.8.1.4 3.38
-
 Lipoamide
-
 Glycine max

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.6.2.6 mitochondrion
-
 Glycine max 5739
-
1.8.1.4 mitochondrion
-
 Glycine max 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.6.2.6 50030
-
 electrospray mass spectrometry Glycine max
1.8.1.4 50027
-
 x * 50027, MW only of protein, flavin is reduced during analysis, electrospray MS analysis Glycine max

Organism

EC Number Organism UniProt Comment Textmining
1.6.2.6 Glycine max O81413
-
-
1.8.1.4 Glycine max O81413
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.6.2.6
-
 Glycine max
1.8.1.4
-
 Glycine max

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.6.2.6 leaf
-
 Glycine max
-
1.6.2.6 root
-
 Glycine max
-
1.6.2.6 root nodule
-
 Glycine max
-
1.6.2.6 stem
-
 Glycine max
-
1.8.1.4 nodule enzyme form FlbR-2 Glycine max
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.8.1.4 83.06
-
 lipoamide reduction Glycine max

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.6.2.6 NADH + ferrileghemoglobin
-
 Glycine max NAD+ + ferroleghemoglobin
-
 ?
1.6.2.6 NADH + lipoamide
-
 Glycine max NAD+ + ?
-
 ?
1.8.1.4 dihydrolipoamide + NAD+
-
 Glycine max lipoamide + NADH
-
 ?
1.8.1.4 ferrileghemoglobin + NADH + H+
-
 Glycine max ferroleghemoglobin + NAD+
-
 r
1.8.1.4 lipoamide + NADH
-
 Glycine max dihydrolipoamide + NAD+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.8.1.4 dimer x * 50027, MW only of protein, flavin is reduced during analysis, electrospray MS analysis Glycine max

Synonyms

EC Number Synonyms Comment Organism
1.6.2.6 FLbR-2
-
 Glycine max

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.6.2.6 1.6
-
 ferrileghemoglobin recombinant FLbR-2 Glycine max
1.6.2.6 137
-
 NADH recombinant FLbR-2 Glycine max
1.6.2.6 392
-
 Lipoamide recombinant FLbR-2 Glycine max

Cofactor

EC Number Cofactor Comment Organism Structure
1.6.2.6 FAD not covalently bound to the protein Glycine max
1.8.1.4 FAD covalently bound to the protein Glycine max
1.8.1.4 NAD+
-
 Glycine max
1.8.1.4 NADH
-
 Glycine max

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.6.2.6 Glycine max isoelectric focusing, pH-range 4.0-6.5
-
 5.8