BRENDA - Enzyme Database show

Isolation und Eigenschaften von Liponsäuredehydrogenase aus Chloroplasten

Jacobi, G.; Ă–hlers, U.; Z. Pflanzenphysiol. 58, 193-206 (1968)
No PubMed abstract available

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
1.8.1.4
cysteine
NADH-oxidation with free lipoic acid is strongly dependent on the addition of NAD+, EDTA, Mg2+ and cysteine, the reverse reaction with reduced lipoic acid and NAD+ does not show any requirement for cofactors
Spinacia oleracea
1.8.1.4
EDTA
NADH-oxidation with free lipoic acid is strongly dependent on the addition of NAD+, EDTA, Mg2+ and cysteine, the reverse reaction with reduced lipoic acid and NAD+ does not show any requirement for cofactors
Spinacia oleracea
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.8.1.4
arsenite
0.3 mM, 50% inhibition
Spinacia oleracea
1.8.1.4
additional information
no inhibition by iodoacetate
Spinacia oleracea
1.8.1.4
PCMB
0.1 mM, 50% inhibition
Spinacia oleracea
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.8.1.4
0.0215
-
NADH
-
Spinacia oleracea
1.8.1.4
2.1
-
lipoic acid
-
Spinacia oleracea
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.8.1.4
chloroplast
-
Spinacia oleracea
9507
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.8.1.4
Mg2+
NADH-oxidation with free lipoic acid is strongly dependent on the addition of NAD+, EDTA, Mg2+ and cysteine, the reverse reaction with reduced lipoic acid and NAD+ does not show any requirement for cofactors
Spinacia oleracea
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.8.1.4
Spinacia oleracea
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.8.1.4
-
Spinacia oleracea
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.8.1.4
leaf
-
Spinacia oleracea
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.8.1.4
2 ferricyanide + NADH
-
394001
Spinacia oleracea
2 ferrocyanide + NAD+ + H+
-
-
-
?
1.8.1.4
dihydrolipoamide + NAD+
-
394001
Spinacia oleracea
lipoamide + NADH
-
-
-
r
1.8.1.4
oxidized 2,6-dichlorophenolindophenol + NADH
-
394001
Spinacia oleracea
? + NAD+
-
-
-
?
1.8.1.4
vitamin K5 + NADH
-
394001
Spinacia oleracea
?
-
-
-
?
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.8.1.4
NAD+
NADH-oxidation with free lipoic acid is strongly dependent on the addition of NAD+, EDTA, Mg2+ and cysteine, the reverse reaction with reduced lipoic acid and NAD+ does not show any requirement for cofactors
Spinacia oleracea
1.8.1.4
NADH
-
Spinacia oleracea
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
1.8.1.4
cysteine
NADH-oxidation with free lipoic acid is strongly dependent on the addition of NAD+, EDTA, Mg2+ and cysteine, the reverse reaction with reduced lipoic acid and NAD+ does not show any requirement for cofactors
Spinacia oleracea
1.8.1.4
EDTA
NADH-oxidation with free lipoic acid is strongly dependent on the addition of NAD+, EDTA, Mg2+ and cysteine, the reverse reaction with reduced lipoic acid and NAD+ does not show any requirement for cofactors
Spinacia oleracea
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.8.1.4
NAD+
NADH-oxidation with free lipoic acid is strongly dependent on the addition of NAD+, EDTA, Mg2+ and cysteine, the reverse reaction with reduced lipoic acid and NAD+ does not show any requirement for cofactors
Spinacia oleracea
1.8.1.4
NADH
-
Spinacia oleracea
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.8.1.4
arsenite
0.3 mM, 50% inhibition
Spinacia oleracea
1.8.1.4
additional information
no inhibition by iodoacetate
Spinacia oleracea
1.8.1.4
PCMB
0.1 mM, 50% inhibition
Spinacia oleracea
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.8.1.4
0.0215
-
NADH
-
Spinacia oleracea
1.8.1.4
2.1
-
lipoic acid
-
Spinacia oleracea
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.8.1.4
chloroplast
-
Spinacia oleracea
9507
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.8.1.4
Mg2+
NADH-oxidation with free lipoic acid is strongly dependent on the addition of NAD+, EDTA, Mg2+ and cysteine, the reverse reaction with reduced lipoic acid and NAD+ does not show any requirement for cofactors
Spinacia oleracea
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.8.1.4
-
Spinacia oleracea
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.8.1.4
leaf
-
Spinacia oleracea
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.8.1.4
2 ferricyanide + NADH
-
394001
Spinacia oleracea
2 ferrocyanide + NAD+ + H+
-
-
-
?
1.8.1.4
dihydrolipoamide + NAD+
-
394001
Spinacia oleracea
lipoamide + NADH
-
-
-
r
1.8.1.4
oxidized 2,6-dichlorophenolindophenol + NADH
-
394001
Spinacia oleracea
? + NAD+
-
-
-
?
1.8.1.4
vitamin K5 + NADH
-
394001
Spinacia oleracea
?
-
-
-
?